WO2010031772A3 - Alternative butanol production process in a microbial cell - Google Patents
Alternative butanol production process in a microbial cell Download PDFInfo
- Publication number
- WO2010031772A3 WO2010031772A3 PCT/EP2009/061961 EP2009061961W WO2010031772A3 WO 2010031772 A3 WO2010031772 A3 WO 2010031772A3 EP 2009061961 W EP2009061961 W EP 2009061961W WO 2010031772 A3 WO2010031772 A3 WO 2010031772A3
- Authority
- WO
- WIPO (PCT)
- Prior art keywords
- microbial cell
- alternative
- coa
- production process
- butanol
- Prior art date
Links
Classifications
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/0004—Oxidoreductases (1.)
- C12N9/0006—Oxidoreductases (1.) acting on CH-OH groups as donors (1.1)
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/0004—Oxidoreductases (1.)
- C12N9/0008—Oxidoreductases (1.) acting on the aldehyde or oxo group of donors (1.2)
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/0004—Oxidoreductases (1.)
- C12N9/0051—Oxidoreductases (1.) acting on a sulfur group of donors (1.8)
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/10—Transferases (2.)
- C12N9/1022—Transferases (2.) transferring aldehyde or ketonic groups (2.2)
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/10—Transferases (2.)
- C12N9/1025—Acyltransferases (2.3)
- C12N9/1029—Acyltransferases (2.3) transferring groups other than amino-acyl groups (2.3.1)
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P7/00—Preparation of oxygen-containing organic compounds
- C12P7/02—Preparation of oxygen-containing organic compounds containing a hydroxy group
- C12P7/04—Preparation of oxygen-containing organic compounds containing a hydroxy group acyclic
- C12P7/16—Butanols
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02E—REDUCTION OF GREENHOUSE GAS [GHG] EMISSIONS, RELATED TO ENERGY GENERATION, TRANSMISSION OR DISTRIBUTION
- Y02E50/00—Technologies for the production of fuel of non-fossil origin
- Y02E50/10—Biofuels, e.g. bio-diesel
Abstract
The present invention relates to a microbial cell comprising genes encoding enzymes that catalyse the following reactions a) pyruvate to acetolactate, b) acetolactate to 2,3-dihydroxyisovalerate, c) 2,3-dihydroxyisovalerate to 2-ketoisovalerate, d) 2- ketoisovalerate to isobutyryl-CoA, e) isobutyryl-CoA to butyryl-CoA, f) butyryl-CoA to butyraldehyde, and g) butyraldehyde to butanol, wherein the cell produces butanol. The invention further relates to a process for the production of butanol wherein the microbial cell according to the present invention is used.
Applications Claiming Priority (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
EP08164412 | 2008-09-16 | ||
EP08164412.2 | 2008-09-16 |
Publications (2)
Publication Number | Publication Date |
---|---|
WO2010031772A2 WO2010031772A2 (en) | 2010-03-25 |
WO2010031772A3 true WO2010031772A3 (en) | 2010-06-10 |
Family
ID=40326716
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
PCT/EP2009/061961 WO2010031772A2 (en) | 2008-09-16 | 2009-09-15 | Alternative butanol production process in a microbial cell |
Country Status (1)
Country | Link |
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WO (1) | WO2010031772A2 (en) |
Families Citing this family (4)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
BR112012020261B8 (en) | 2010-02-12 | 2020-04-14 | California Inst Of Techn | recombinant saccharomyces cerevisiae and isobutanol production method |
CN102174557A (en) * | 2011-03-16 | 2011-09-07 | 江苏大学 | Recombinant spores of surface displayed silkworm alcohol dehydrogenases and preparation method of same |
WO2013175428A2 (en) * | 2012-05-23 | 2013-11-28 | Cellworks Research India Pvt. Ltd | A genetically modified microorganism, a process and methods for production of isobutanol |
CN102732502B (en) * | 2012-06-04 | 2014-07-09 | 东北农业大学 | Marker-free lactic acid bacterium genome gene operation system and its application |
Citations (6)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
WO2007041269A2 (en) * | 2005-09-29 | 2007-04-12 | E. I. Du Pont De Nemours And Company | Fermentive production of four carbon alcohols |
WO2007050671A2 (en) * | 2005-10-26 | 2007-05-03 | E. I. Du Pont De Nemours And Company | Fermentive production of four carbon alcohols |
WO2007130518A2 (en) * | 2006-05-02 | 2007-11-15 | E. I. Du Pont De Nemours And Company | Fermentive production of four carbon alcohols |
WO2008052991A2 (en) * | 2006-10-31 | 2008-05-08 | Dsm Ip Assets B.V. | Butanol production in a eukaryotic cell |
WO2008074794A1 (en) * | 2006-12-19 | 2008-06-26 | Dsm Ip Assets B.V. | Butanol production in a prokaryotic cell |
WO2008098227A2 (en) * | 2007-02-09 | 2008-08-14 | The Regents Of The University Of California | Biofuel production by recombinant microorganisms |
-
2009
- 2009-09-15 WO PCT/EP2009/061961 patent/WO2010031772A2/en active Application Filing
Patent Citations (6)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
WO2007041269A2 (en) * | 2005-09-29 | 2007-04-12 | E. I. Du Pont De Nemours And Company | Fermentive production of four carbon alcohols |
WO2007050671A2 (en) * | 2005-10-26 | 2007-05-03 | E. I. Du Pont De Nemours And Company | Fermentive production of four carbon alcohols |
WO2007130518A2 (en) * | 2006-05-02 | 2007-11-15 | E. I. Du Pont De Nemours And Company | Fermentive production of four carbon alcohols |
WO2008052991A2 (en) * | 2006-10-31 | 2008-05-08 | Dsm Ip Assets B.V. | Butanol production in a eukaryotic cell |
WO2008074794A1 (en) * | 2006-12-19 | 2008-06-26 | Dsm Ip Assets B.V. | Butanol production in a prokaryotic cell |
WO2008098227A2 (en) * | 2007-02-09 | 2008-08-14 | The Regents Of The University Of California | Biofuel production by recombinant microorganisms |
Non-Patent Citations (5)
Title |
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ATSUMI SHOTA ET AL: "Non-fermentative pathways for synthesis of branched-chain higher alcohols as biofuels", NATURE, vol. 451, no. 7174, 1 January 2008 (2008-01-01), pages 86 - 90, XP002491157, ISSN: 0028-0836 * |
GIRBAL L ET AL: "Regulation of solvent production in Clostridium acetobutylicum", TRENDS IN BIOTECHNOLOGY, vol. 16, no. 1, 1 January 1998 (1998-01-01), pages 11 - 16, XP004101891, ISSN: 0167-7799 * |
JONES D T ET AL: "Acetone-butanol fermentation revisited", MICROBIOLOGICAL REVIEWS, vol. 50, no. 4, 1 December 1986 (1986-12-01), pages 484 - 524, XP002425366, ISSN: 0146-0749 * |
RATNATILLEKE ANANDA ET AL: "Cloning and sequencing of the coenzyme B12-binding domain of isobutyryl-CoA mutase from Streptomyces cinnamonensis, reconstitution of mutase activity, and characterization of the recombinant enzyme produced in Escherichia coli", JOURNAL OF BIOLOGICAL CHEMISTRY, vol. 274, no. 44, 29 October 1999 (1999-10-29), pages 31679 - 31685, XP002460830, ISSN: 0021-9258 * |
WOODS D R: "The genetic engineering of microbial solvent production", TRENDS IN BIOTECHNOLOGY, vol. 13, no. 7, 1 July 1995 (1995-07-01), pages 259 - 264, XP004207180, ISSN: 0167-7799 * |
Also Published As
Publication number | Publication date |
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WO2010031772A2 (en) | 2010-03-25 |
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