WO2004028502A1

WO2004028502A1 - Cleansing composition containing antifungal agent and antibacterial agent

Info

Publication number: WO2004028502A1
Application number: PCT/JP2003/012325
Authority: WO
Inventors: Shigenobu Kitsugi; Noriyuki Komatsu
Original assignee: Mochida Pharmaceutical Co., Ltd.
Priority date: 2002-09-27
Filing date: 2003-09-26
Publication date: 2004-04-08
Also published as: AU2003266643A1; KR101045867B1; CN100370965C; JPWO2004028502A1; KR20050059141A; CN1684662A

Abstract

It is intended to provide a cleansing composition for drugs, quasi drugs or cosmetics which has broad antibacterial and antifungal spectra and is excellent in efficacy and safety. Namely, a cleansing composition for drugs, quasi drugs or cosmetics which contains an azole-type antifungal agent and an antibacterial agent. As the azole-type antifungal agent as described above, miconazole nitrate is particularly preferable.

Description

Description Cleaning composition containing antifungal agent and antibacterial agent

The present invention relates to a cleaning composition for pharmaceuticals, quasi-drugs or cosmetics, which has a broad antibacterial and antifungal spectrum and is excellent in efficacy and safety. Background art

The most common method of daily hand washing is with stones and running water, which is the physical removal of dirt, organic matter and transient microorganisms (bacteria, fungi, viruses, etc.) from the hands. . In experiments to examine the germicidal effect of hand washing, the bactericidal effect of bacteria that have been artificially attached to hands depends on the time of hand washing, and the amount of bacteria is 1/10 in 15 seconds of hand washing. It has been reported that 1Z100 in 30 seconds and 11000 in more than 1 minute. In other words, by washing with ordinary stone stones and running water for 1 minute or more, the attached bacteria can be sufficiently removed. However, in daily hand washing, it is very difficult to secure a hand washing time of 1 minute or more.

For this reason, antibacterial agent-containing stones for increasing the disinfection efficiency during washing are commercially available. Used by workers in food production and medical facilities, where bacterial infection is particularly important, to prevent food poisoning, disinfect and disinfect bacteria from the environment that adhere to soil or pets, etc. In addition, cleaning with antibacterial agent-containing stones in ordinary households is becoming commonplace. As such an antibacterial agent-containing stone, an antibacterial agent-containing stone containing triclosan, triclocarban and an antibacterial agent such as Z or isopropylmethylphenol is commercially available.

Some compositions containing an antifungal agent for preventing dandruff are known. There is a disclosure of an anti-dandruff agent containing miconazole or its nitrate, which has a strong antifungal effect against fungi and ora / e (Japanese Patent Application Laid-Open No. 8-3042). The inhibitor used herein is, for example, shampoo for preventing dandruff caused by P. ovale, but the inhibitor does not contain the antibacterial agent as described above.

Some disclose compositions containing one or more antibacterial and antifungal agents and phospholipids that inhibit the biosynthesis of fungi and mold ergosterol (Table 2) Report). In the literature, azole which is an antifungal agent is exemplified as an antibacterial and antifungal agent, and a compound known as an antibacterial agent is not exemplified. Judging from the statement that it is effective against dermatophyte fungi, the bacteria in this document substantially means fungi and do not include antibacterial agents.

Some actives, along with certain surfactants, disclose foaming cleansing creams for greasy or acne-prone skin that include antiseborrheic actives and Z or antimicrobial agents. Japanese Patent Application Laid-Open No. 2002-14045 736). In the literature, among the exemplified groups of the above-mentioned bacilli, miconazole and its hydrochloride, which are antifungals, as well as antibiotics are also exemplified. It states that two or more antimicrobial agents may be used, but there is no particular indication that two or more antimicrobial agents should be used. A fungicide composition having a skin-protecting effect containing a complex of a fungicide and a water-swellable viscosity mineral has also been proposed (Japanese Patent Application Laid-Open No. 10-265408). In the literature, there are fungicides Antibacterial agents such as triclosan and isopropylmethylphenol, azolic antifungal agents and the like are described. In addition, the document states that when a fungicide is used in a complex with the above viscous mineral, the antibacterial activity is persistent, and that one or more fungicides are arbitrarily selected. However, the examples exemplified for each application show the sustained effect when one kind of drug corresponding to each application is formed into a complex with the above-mentioned viscous mineral.

The above documents do not indicate the necessity of using a combination of an antibacterial agent and an antifungal agent.

Human skin usually contains a large number of indigenous and transmissible bacteria. For example, S. aureus is the most frequent causative bacterium for skin infections, and routinely seen diseases include rash (bullous impetigo). T. nibrum and T. mentagrophytes are fungi that cause dermatophytosis (ringworm). Tinea pedis (athlete's foot) has weak subjective symptoms, except in severe cases, and lacks sufficient awareness that it is an infectious disease that infects others, and stops treatment when the subjective symptoms disappear (poor compliance) Therefore, there are many chances of infection from bathroom mats and slippers. It has been reported that athlete's foot may develop depending on the host even with a small amount of bacteria.

In addition to skin diseases, fungi attached to the hands can enter the body orally, causing fungal infections in various organs.

Fungi are also considered as one of the causes (allergens) of allergic diseases, and C. albicans is regarded as important in relation to atopic dermatitis as an allergic skin disease. .

In recent years, changes in living environment, daily use of drugs such as antibiotics and steroids However, opportunistic infections caused by a decrease in the immune function of the host are also viewed as a problem. From the standpoint of preventing opportunistic infections, the importance of removing bacteria as well as bacteria is important for preventing infection by washing the skin !!

However, when the antifungal effect of the conventional antibacterial agent-containing stones was examined, it was found that the antifungal effect on T. rubrum or the like was scarce or insufficient. These stone tests with antibacterial agents are mainly aimed at preventing food poisoning and disinfecting and disinfecting environmentally-derived bacteria. In fact, in particular, the antifungal effect on fungi attached to the skin has not been regarded as very important.

In view of the circumstances described above, the present inventors have recognized the need for a cleaning composition having a broad antibacterial and antifungal spectrum.

Based on this recognition, the inventor of the present invention, in the process of variously examining a cleaning composition containing an antifungal agent and an antibacterial agent, found that among the antifungal agents, this was used as a normal antibacterial stone. It was found that there is a problem that even if it is blended as it is, its solubility, effect, stability, and the like vary greatly, and it is difficult to obtain a stable cleaning composition.

In addition, some of the antifungals, antibacterials, and cleaning ingredients have a skin irritating effect, and their effects (antifungal, antibacterial, cleaning, It is also necessary to balance the balance between antibacterial agents and residual effects of antifungal agents) and safety (less skin irritation). Disclosure of the invention

The present invention solves these problems and provides a cleaning composition containing an antifungal agent and an antibacterial agent, particularly a cleaning composition that can be used for pharmaceuticals, quasi-drugs, or cosmetics. It has a wide spectrum of antibacterial and antifungal properties, is stable and has excellent usability, has excellent detergency, has little skin irritation, and has the effect of preventing itching and deodorizing of the scalp and body. An object of the present invention is to provide a cleaning composition capable of preventing infectious diseases caused by shampoo.

The present inventors have conducted intensive studies on a cleaning composition containing an antifungal agent and an antibacterial agent, and found that a cleaning composition containing an azole-based antifungal agent and an antibacterial agent has a strong and broad It has an antibacterial and antifungal spectrum, the product itself is stable, it has excellent usability, it has excellent detergency, it does not cause skin irritation or other adverse events, and it can prevent itching and deodorant effects on the scalp and body. The present inventors have found that at least one of the above-mentioned problems can be solved, such as an effect of preventing infectious diseases caused by indigenous bacteria and passing bacteria, and thus completed the present invention.

According to the present invention, there is provided a cleaning composition for a pharmaceutical, a quasi-drug, or a cosmetic, which contains an azole antifungal agent and an antibacterial agent.

In the cleaning composition, the antifungal agent is preferably miconazole and / or a salt thereof. The antibacterial agent is preferably at least one selected from triclosan, triclocarban and isopropylmethylphenol.

A cleaning composition in which the antifungal agent is miconazole nitrate and the antibacterial agent is Tricsan is a preferred embodiment of the present invention. In the cleaning composition of the present invention, usually, the content of the antifungal agent is 0.1 to 2.0 wt%, and the content of the antibacterial agent is 0.01 to 1.5 wt%. It is.

The cleaning composition of the present invention may contain a mild surfactant as a cleaning component.

The washing and cleaning composition of the present invention is provided, for example, in the form of stone. BRIEF DESCRIPTION OF THE FIGURES

FIG. 1 is a graph showing the results of a bacterial test in Example 5, showing the cleaning effect of the stone of the present invention in comparison with a control stone.

FIG. 2 is a graph showing the results of a fungal test in Example 5, showing the cleaning effect of the stone of the present invention in comparison with a control stone. BEST MODE FOR CARRYING OUT THE INVENTION

Hereinafter, the present invention will be described in detail.

The present invention is an antifungal 'antibacterial cleaning composition for pharmaceuticals, quasi-drugs or cosmetics, having a broad antifungal and antibacterial spectrum. The cleaning composition of the present invention contains an antifungal agent and an antibacterial agent as essential components, and is characterized by containing an azole antifungal agent as the antifungal agent.

Examples of the azolic antifungal agent in the present invention include miconazole, clotrimazole, econazole, choconazole, oxiconazole, fluconazole, itraconazole, ketoconazole, croconazole, neticonconazole, and isoconazole. Nazole, sulconazole, bifonazole and their salts You. Examples of the salt include nitrate and hydrochloride, and preferably nitrate. In the present invention, two or more of these may be included in the cleaning composition. Of these, miconazole, clotrimazole, econazole, choconazole, oxyconazole, sulconazole, bifonazole and their salts are preferred, and miconazole nitrate is more preferred. It is.

In the present specification, the above-mentioned miconazole and miconazole nitrate mean a compound having a structure represented by the following (I) and (Π).

(I) Miconazole (ID

The above azole antifungal agent is available as a commercial product.

The antibacterial agent used in the present invention is not particularly limited as long as it has a growth inhibitory effect on bacteria, but a diphenyl ether compound, a carbanilide compound or a phenol compound is preferable, and specifically, triclosan, triclocarban And isopropylmethylphenol. Among them, Trik mouth sun is preferred.

These antibacterial agents are available as commercial products.

A particularly preferred embodiment of the present invention is the combination, wherein the antifungal agent is miconazole nitrate and the antibacterial agent is Tricsan.

The amount of the antifungal agent and the antibacterial agent in the composition is not particularly limited, but is usually The compounding amount of the antifungal agent is 0.1 to 2.0%, and the compounding amount of the antibacterial agent is 0.0 :! ~ 1.5 wt%. Specifically, when the antifungal agent is miconazole nitrate, the amount is preferably 0.1 to 1.0 wt%, and when the antibacterial agent is triclosan, the amount is 0.05 to 0.5 wt%. 1.0% is preferred.

The mixing ratio of the antifungal agent to the antibacterial agent is not particularly limited, but is usually 1: 5 to 5: 1, preferably 1: 5 to 5: 1, and preferably 1: 1 to 5: 1, more preferably 1: 1 to 2.5: 1, and still more preferably antifungal agent: antibacterial agent = 2: 1 to 2.5: 1. Specifically, a particularly preferable combination of the antifungal agent, the antibacterial agent, and the combination ratio thereof is such that the antifungal agent is miconazole nitrate, the antibacterial agent is triclosan, and the combination ratio is miconazole nitrate. : Triclosan = 2.5: 1.

In the present invention, the cleaning component to be added to the cleaning composition is not particularly limited as long as the cleaning component does not impair the efficacy of the antifungal agent and the antibacterial agent, and may include an anionic surfactant. Is preferred. Specifically, anionic surfactants such as alkyl sulfate sodium salt, amphoteric surfactants such as alkyl dimethyl ammonium betaine, and nonionic surfactants such as polyoxyethylene alkyl ether Surfactants and the like can be used in appropriate combination.

These surfactants are preferably low irritants, and examples of low irritant anionic surfactants include monoalkyl phosphates, acyl glutamates, acyl methyl taurates, cocoyl isethionates, Lauroyl-methyl | 8-alanine salt, etc. Examples of the low-irritating amphoteric surfactant include betaine-type amphoteric surfactants such as 2-alkyl-1-N-carboxymethyl_N-hydroxy. And ethyl imidazolidine betaine. A combination of a mildly anionic surfactant and a mildly amphoteric surfactant is preferred.

The cleaning composition of the present invention further includes components used in ordinary cleaning compositions, for example, humectants such as propylene glycol and glycerin, conditioning components such as cationic polymers, coloring agents such as pigments and pigments, Viscosity modifiers such as methylcellulose and polyethylene glycol, pH regulators such as citric acid and potassium oxide, salts such as sodium chloride, plant extracts, preservatives, vitamins, fragrances, ultraviolet absorbers, antioxidants , Water and the like can be appropriately added.

In the present specification, the term “cleaning composition” refers to a composition in which a cleaning component is blended, and after use, is washed away with running water or the like. Although the form is not particularly limited, stone (solid stone, liquid stone, foamed stone, body soap, hand soap, etc.), cleansing foam, shampoo (hair shampoo, dry shampoo, etc.) And the like. Of these, stones are preferred, and liquid stones and body soaps are particularly preferred. Examples of the cleaning composition of the present invention are shown below, but the present invention is not limited thereto.

• (Example 1) Antibacterial and antifungal activities of miconazole nitrate and triclosan at //?

[Target strain]

Stapnylococcus aureus ATCC 29213 Escherichia coli ATCC 25922

Candida albicans IFM 40213

Trichophyton rubrim IFM 45626

[Measurement of MIC value]

(1) After culturing on Mueller Hinton II agar medium (BBL) at 35 ° C for 18-20 hours, it was adjusted to McFarland ΝαΟ.5 with sterile physiological saline. Then, it was diluted 10-fold with sterile physiological saline to obtain a test bacterial solution.

(2) The test drug was dissolved in DMSO, and miconazole was adjusted to 102,400 Mg / mL (titer) and triclosan was adjusted to 409,600 ^ g / mL (titer).

The miconazole solution prepared at 102, 400 / mL (titer) was further prepared in a two-fold dilution series using DMS0 to prepare 102, 400 to 50 wgZmL (titer).

Triclosan drug solution adjusted to 409, 600 M g / mL (titer) was further prepared in a 2-fold dilution series using DMSO, and adjusted to 409, 600 to 6.00 g / mL (titer). .

(3) Cation adjustment of each dilution series of each drug prepared in (2), so that miconazole is 1,024-0.5 gZmL and triclosan is 4,096-0.06 gZmL. Was added to the obtained Mueller-Hinton broth (CAMHB) (at this time, the final DMSO concentration was adjusted to 2% or less).

(4) Each 100 L of the measurement medium prepared in (3) was dispensed. As a control 100 L of the drug-free medium was dispensed.

(5) The inoculum was inoculated into each well at 5 / L, and the amount of bacteria in the drug-free medium used as a target was measured to determine the amount of inoculated bacteria.

(6) After confirming the growth of the bacteria in the drug-free medium used as a control, the minimum drug concentration in the tube where growth of the bacteria was not visually observed was defined as MIC.

(1) C. albicans was cultured on Sabouraud dextrose agar medium (BBL) at 35 ° C. for 24 to 48 hours, and then adjusted to McFarland ΝαΟ.5 with sterile physiological saline. It was diluted 1000-fold with RPMI 1640 medium containing 0.165 mol / L MOPS to obtain a test bacterial solution (1 to 5 × 10 ³ cells / mL).

T. rubrum was modified 2% on a modified 1Z10 Sabouraud glucose agar slant. Then, after culturing for 1 to 2 weeks, sterile physiological saline solution containing 0.1% Tween 80 was added, conidia were collected, and the conidia were filtered with sterile gauze. The conidia suspension was further diluted with sterile physiological saline containing 0.1% Tween 80, and the number of bacteria was adjusted to 10 ⁶ cellsZmL using a hemocytometer to obtain a test bacterial solution.

(2) The drug to be measured was dissolved in DMSO and adjusted to 12,800 Mg / mL (titer) for each of miconazole and triclosan. The miconazole and triclosan drug solutions prepared at 12,800 / xg / mL (titer) were further prepared in 2-fold dilution series using DMSO to obtain 12,800-60 g / mL (titer). It was adjusted.

(3) The dilution series of each drug prepared in (2) was adjusted to 128-0.06 g / mL in RPMI 1640 medium containing 0.165 mol / LM OPS. (At this time, the final DMSO concentration was adjusted to 2% or less).

(4) 100 L of the culture solution prepared in (2) was inoculated to 100 L of the measurement medium prepared in (3), C. albicans was inoculated at 35 ° C for 24-48 hours, and T. rubrum was Cultured at 27 for 1-2 weeks. However, the cultivation time was appropriately extended depending on the growth of the bacteria in the drug-free medium. As a control, 100 L of a drug-free medium was dispensed.

(5) After confirming the growth of the bacteria in the drug-free medium used as a control, the minimum drug concentration in the tube where growth of the bacteria was not visually observed was defined as MIC.

(MIC value g / mL) The evaluation results are shown in Table 1. table 1

In the table, evaluation MIC value (g / mL)

<0.1

〇 0.1 to less than 1

Δ1 ~: Less than L0

X 10 ≤ Trik mouth sun showed a strong growth inhibitory effect on bacteria, and miconazole nitrate showed a strong growth inhibitory effect on fungi. It was found that by mixing both, a powerful and broad antifungal and antibacterial spectrum could be obtained. ' (Example 2) Preparation example of liquid stone (1)

In this OP 00 g

Triclosan 0.30 g

0.75 g of miconazole nitrate

Lauroylmethyl-3-alanan sodium 12.00 g

2-alkyl-1-N-hydroxypropyloxy-N-hydroxyethylimidazolinium betaine 12.00 g

Propylene glycol 8.00 g

Antioxidant appropriate amount

pH adjuster qs

purified water

(Example 3) Stability test of liquid stone

The liquid stone prepared in Example 2 was a clear, slightly yellow liquid immediately after preparation, and no insolubles were observed. In addition, this liquid stone was stored in a plastic container for 6 months at a temperature of 40 ° C and a relative humidity of 75%. As a result, the residual ratio of miconazole nitrate and triclosan was 95% or more in each case. Quantified by the usual method used), and no precipitation, coloring, etc. were observed. (Example 4) Use of liquid stone (1)

The liquid stone prepared in Example 2 was hand-washed and used for the whole body according to a conventional method. Uncomfortable feeling during and after washing, tingling of skin, and bulkiness of skin are observed. It had excellent usability.

After washing with this liquid stone, the palms and soles were imprinted on the agar medium (stamp method), and cultivation was performed according to a conventional method. As a control, a similar test was carried out using a commercially available antibacterial stone. The number of bacteria and fungi detected after culturing was clearly lower when the liquid stone of the present invention was used than when a commercially available antibacterial stone was used.

(Example 5) Use of liquid stone (2)

[Method]

Using the liquid stone prepared in Example 2, the following comparative test was performed.

1. Bacterial test

(1-1) Using a stamp-stamp medium (commercially available SCDLP agar medium; containing soybean casein hydrolyzate, lecithin and polysorbate 80), pre-test bacteria were collected from test subjects (N = 5) . Hand washing was prohibited 5 hours before the test.

(1-2) Liquid stone prepared in Example 2 diluted to 7% and placed in a foam ejection container (invention stone) and liquid stone prepared by removing miconazole nitrate from the composition of Example 2 (control stone) ), The subject's right and left palms were washed with one hand for a certain period of time.

Washing was carried out by each person in charge of the present invention stones and the control stones, washing the hands of all subjects randomly under the blinds, left and right randomly (2 pushes for 5 seconds). .

(1-3) The detergent was rinsed with tap water (e LZmin) for a certain period of time (5 seconds: 50 O mL).

(1-4) Both hands were dried with a dryer (cold air), and the bacteria were collected again. (1-5) The bacteria were collected again 5 hours after washing without washing hands.

(1-6) The medium from which the bacteria were collected was cultured in an incubator at 35 ° C for 24 hours. '(1-7). A photograph of the detected medium was taken, and the number of bacterial colonies was measured.

2. Fungal test

(2-1) Use the media for foot press (15 g of Bactogaga, 10 g of neopeptone, 20 g of glucose, 100 Omg of cycloheximide, 5 Omg of chloramphenicol, 100 mg of genyumycin sulfate, 100 OmL of distilled water) Before the test, the fungi on the feet of the subjects (N = 5) were collected.

(2-2) Using the stone of the present invention 対照 and the control stone 希釈 diluted to 7% and placed in a foam ejection container, the right sole and the left sole of the subject were washed with one foot for a certain period of time.

Washing was carried out by the person in charge of the stone of the present invention and the control stone by washing the feet of all subjects randomly under the blind, left and right with a fixed method (2 pushes for 5 seconds). .

(2-3) The cleaning agent was rinsed with tap water (6 LZmin) for a certain period of time (5 seconds: 50 OmL).

(2-4) Both feet were dried with a dryer (cold air), and the bacteria were collected again.

(2-5) The culture medium from which the bacteria were collected was cultured in an incubator at 27 ° C for 7 to 14 days. (2-6) A photograph of the detected medium was taken, and the number of fungal colonies was counted.

[Result]

The results are shown in Figure 1 (bacteria) and Figure 2 (fungi).

As shown in Fig. 1, when the stone of the present invention was used, the average number of bacterial colonies (expressed as% with 100% before washing) showed a marked decrease immediately after washing, and 5 hours after washing. The value was lower than before cleaning. On the other hand, in Control Stone I, the number of colonies temporarily decreased immediately after washing, but the value 5 hours after washing was higher than that before washing.

As shown in FIG. 2, when the stone of the present invention was used, the average number of fungal colonies (expressed as 100% before washing) was markedly decreased immediately after washing. On the other hand, the control stone No. showed no decrease in the number of colonies immediately after washing, and on the contrary, the value was higher than that before washing.

From the above, it was confirmed that the stone of the present invention was superior to the control stone in antibacterial and antifungal activities.

(Example 6) In vitro antibacterial and antifungal activities of a mixed solution of miconazole nitrate and triclosan

[Target strain]

Staphylococcus aureus ATCC 29213,

Escherichia coli ATCC 25922

Candida albicans IFM 40213

Trichophyton rubruin IFM 45626

[Drug mix ratio]

(1) Miconazole nitrate: Triclosan = 2.5

(0.710g: 0.2863g) (2) Miconazole nitrate: Triclosan = 2: 1

(0.6634 g: 0.3340 g)

(3) Miconazole nitrate: Triclosan = 1: 1

(0.4975 g: 0.5010 g)

[Drug preparation method]

(1) Miconazole nitrate and triclosan were weighed so as to be 1 g at the above-mentioned drug compounding ratio.

(2) This 1 g was dissolved in 39.06 mL of dimethyl sulfoxide (DMSO) to prepare a solution of 25,600 gZmL.

(3) A 25, 60 O ^ gZmL solution was used as a stock solution for the following tests.

[Measurement of MIC value]

(1) After culturing on a Myura-Hinton II agar medium (BBL) at 35 ° (18 hours), adjusted to McFarland ΝαΟ.5 with sterile physiological saline. Then, dilute 10-fold with sterile physiological saline. And used as a test bacterial solution.

(2) The stock solution prepared by the above drug preparation method was diluted 2-fold with DMSO, adjusted to 12,800 gZmL, and then a 2-fold dilution series was prepared with DMSO. It was adjusted to 8 g / mL (titer).

(3) The dilution series of the drug prepared in (2) was added to each cation-adjusted Mueller-Hinton broth (CAMHB) so that the concentration became 128 to 0.008 g / mL. 2% or less).

(5) Inoculated bacterial solution was inoculated into each well in an amount of 5 L, and the amount of bacteria in the drug-free medium used as a control was measured to determine the amount of inoculated bacteria.

(1) C. albicans was cultured on Sabouraud dextrose agar medium (88) at 35 ° (:, 48 hours), and then prepared with sterile physiological saline to McFarland No. 0.5. It was diluted 1000-fold with RPMI 1640 medium containing mol / L MOPS to obtain a test bacterial solution (1 to 5 × 10 ³ cells ZmL).

T. rubrwn was cultured on a modified 1/10 sub-oral glucose agar slant medium for 271 weeks and then added with 0.1% Tween n80 in sterile physiological saline to collect conidia and filtered through sterile gauze. . The conidia suspension was further diluted with RPMI 1640 medium containing 0.165 mol / L MOPS, and the number of bacteria was adjusted to 10 ⁶ cells / mL using a hemocytometer to obtain a test bacterial solution.

(2) A two-fold dilution series was prepared from the stock solution prepared by the above-mentioned drug preparation method using DMSO, and adjusted to 25,600 to 1.5 g / mL (titer).

(3) The dilution series of each drug prepared in (2) was added to RPMI 1640 medium containing 0.165 molZL MOPS to obtain 256-0.015 gZmL (in this case, final DMSO The concentration was adjusted to 2% or less).

(4) The measurement medium prepared in (3) was dispensed in 100 L portions. As a control, 100 L of drug-free medium was dispensed. (5) Inoculate 100 L of the bacterial solution prepared in (1), C. albicans at 35 ° C for 48 hours, and T. rubrim at 21. And cultured for 1 week.

〇 △ X

(6) After confirming the growth of the bacteria in the drug-free medium used as a control, the minimum drug concentration in the tube where growth of the bacteria was not visually observed was defined as MIC. The results of the evaluation of the MI C value (gZmL) are shown in Table 2. Table 2

In the table, the evaluation MIC value (β g / mL)

<0.1

0.1 to less than 1

1 to less than 10

1 0≤

A mixture of miconazole nitrate and triclosan in a ratio of 2.5: 1 to 1: 1 showed a strong growth inhibitory effect against bacteria and fungi. It was found that by mixing both, a strong and broad antifungal and antibacterial spectrum could be obtained. (Example 7) Preparation example of liquid stone (2)

100 g of this product

Triclosan 0.30 g Miconazole nitrate 0.75 g N-coconut oil fatty acid acyl-L-potassium monoglutamate 5.00 g

2-alkyl-1-N-carboxymethyl-1-N-hydroxyethylimidazolinium

10.00 g Propylene glycol 5.00 g Antioxidant

pH regulator

purified water

(Example 8) Preparation example of liquid stone (3)

100 g of this product

Triclocarban 0.50 g Miconazole nitrate 0.75 g

N-lauroylu L- potassium glutamate 30.00 g propylene glycol 3.00 g antioxidant

pH regulator

purified water Industrial applicability

According to the present invention, there is provided the cleaning composition of the present invention for a novel drug, quasi-drug, or cosmetic, which contains an antifungal agent and an antibacterial agent. In particular, cleaning compositions containing an azole antifungal agent and an antibacterial agent as antifungal agents have a strong and broad antibacterial and antifungal spectrum, are stable in the product itself, and have excellent usability. Excellent detergency, no adverse effects such as skin irritation, prevention of scalp and body itch and deodorization effects, prevention of infectious diseases caused by resident bacteria and passing bacteria It has at least one excellent property.

Claims

The scope of the claims

1. A cleaning composition for medicaments, quasi-drugs or cosmetics, which contains an azolic antifungal agent and an antibacterial agent.

2. The cleaning composition according to claim 1, wherein the antifungal agent is miconazole and Z or a salt thereof.

3. The cleaning composition according to claim 1, wherein the antibacterial agent is at least one selected from triclosan, triclocarban, and isopropylmethylphenol. .

4. The cleaning composition according to any one of claims 1 to 3, wherein the antifungal agent is miconazole nitrate, and the antibacterial agent is triclosan.

5. The content of the antifungal agent is 0.1 to 2.01;%, and the content of the antibacterial agent is 0.01 to 1.5 wt%. The cleaning composition according to any one of the above.

6. The cleaning composition according to any one of claims 1 to 5, further comprising a low-irritant surfactant as a cleaning component.

7. The cleaning composition according to any one of claims 1 to 6, wherein the cleaning composition is stone.