WO2010031772A3 - Alternative butanol production process in a microbial cell - Google Patents

Alternative butanol production process in a microbial cell Download PDF

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Publication number
WO2010031772A3
WO2010031772A3 PCT/EP2009/061961 EP2009061961W WO2010031772A3 WO 2010031772 A3 WO2010031772 A3 WO 2010031772A3 EP 2009061961 W EP2009061961 W EP 2009061961W WO 2010031772 A3 WO2010031772 A3 WO 2010031772A3
Authority
WO
WIPO (PCT)
Prior art keywords
microbial cell
alternative
coa
production process
butanol
Prior art date
Application number
PCT/EP2009/061961
Other languages
French (fr)
Other versions
WO2010031772A2 (en
Inventor
Liang Wu
John B. Perkins
Ghislain Schyns
Original Assignee
Dsm Ip Assets B.V.
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Dsm Ip Assets B.V. filed Critical Dsm Ip Assets B.V.
Publication of WO2010031772A2 publication Critical patent/WO2010031772A2/en
Publication of WO2010031772A3 publication Critical patent/WO2010031772A3/en

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0006—Oxidoreductases (1.) acting on CH-OH groups as donors (1.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0008—Oxidoreductases (1.) acting on the aldehyde or oxo group of donors (1.2)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0051—Oxidoreductases (1.) acting on a sulfur group of donors (1.8)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/1022—Transferases (2.) transferring aldehyde or ketonic groups (2.2)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/1025—Acyltransferases (2.3)
    • C12N9/1029—Acyltransferases (2.3) transferring groups other than amino-acyl groups (2.3.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/02—Preparation of oxygen-containing organic compounds containing a hydroxy group
    • C12P7/04—Preparation of oxygen-containing organic compounds containing a hydroxy group acyclic
    • C12P7/16—Butanols
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02E—REDUCTION OF GREENHOUSE GAS [GHG] EMISSIONS, RELATED TO ENERGY GENERATION, TRANSMISSION OR DISTRIBUTION
    • Y02E50/00—Technologies for the production of fuel of non-fossil origin
    • Y02E50/10—Biofuels, e.g. bio-diesel

Abstract

The present invention relates to a microbial cell comprising genes encoding enzymes that catalyse the following reactions a) pyruvate to acetolactate, b) acetolactate to 2,3-dihydroxyisovalerate, c) 2,3-dihydroxyisovalerate to 2-ketoisovalerate, d) 2- ketoisovalerate to isobutyryl-CoA, e) isobutyryl-CoA to butyryl-CoA, f) butyryl-CoA to butyraldehyde, and g) butyraldehyde to butanol, wherein the cell produces butanol. The invention further relates to a process for the production of butanol wherein the microbial cell according to the present invention is used.
PCT/EP2009/061961 2008-09-16 2009-09-15 Alternative butanol production process in a microbial cell WO2010031772A2 (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
EP08164412 2008-09-16
EP08164412.2 2008-09-16

Publications (2)

Publication Number Publication Date
WO2010031772A2 WO2010031772A2 (en) 2010-03-25
WO2010031772A3 true WO2010031772A3 (en) 2010-06-10

Family

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Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/EP2009/061961 WO2010031772A2 (en) 2008-09-16 2009-09-15 Alternative butanol production process in a microbial cell

Country Status (1)

Country Link
WO (1) WO2010031772A2 (en)

Families Citing this family (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR20120129953A (en) 2010-02-12 2012-11-28 캘리포니아 인스티튜트 오브 테크놀로지 Yeast microorganisms with reduced by-product accumulation for improved production of fuels, chemicals, and amino acids
CN102174557A (en) * 2011-03-16 2011-09-07 江苏大学 Recombinant spores of surface displayed silkworm alcohol dehydrogenases and preparation method of same
WO2013175428A2 (en) * 2012-05-23 2013-11-28 Cellworks Research India Pvt. Ltd A genetically modified microorganism, a process and methods for production of isobutanol
CN102732502B (en) * 2012-06-04 2014-07-09 东北农业大学 Marker-free lactic acid bacterium genome gene operation system and its application

Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2007041269A2 (en) * 2005-09-29 2007-04-12 E. I. Du Pont De Nemours And Company Fermentive production of four carbon alcohols
WO2007050671A2 (en) * 2005-10-26 2007-05-03 E. I. Du Pont De Nemours And Company Fermentive production of four carbon alcohols
WO2007130518A2 (en) * 2006-05-02 2007-11-15 E. I. Du Pont De Nemours And Company Fermentive production of four carbon alcohols
WO2008052991A2 (en) * 2006-10-31 2008-05-08 Dsm Ip Assets B.V. Butanol production in a eukaryotic cell
WO2008074794A1 (en) * 2006-12-19 2008-06-26 Dsm Ip Assets B.V. Butanol production in a prokaryotic cell
WO2008098227A2 (en) * 2007-02-09 2008-08-14 The Regents Of The University Of California Biofuel production by recombinant microorganisms

Patent Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2007041269A2 (en) * 2005-09-29 2007-04-12 E. I. Du Pont De Nemours And Company Fermentive production of four carbon alcohols
WO2007050671A2 (en) * 2005-10-26 2007-05-03 E. I. Du Pont De Nemours And Company Fermentive production of four carbon alcohols
WO2007130518A2 (en) * 2006-05-02 2007-11-15 E. I. Du Pont De Nemours And Company Fermentive production of four carbon alcohols
WO2008052991A2 (en) * 2006-10-31 2008-05-08 Dsm Ip Assets B.V. Butanol production in a eukaryotic cell
WO2008074794A1 (en) * 2006-12-19 2008-06-26 Dsm Ip Assets B.V. Butanol production in a prokaryotic cell
WO2008098227A2 (en) * 2007-02-09 2008-08-14 The Regents Of The University Of California Biofuel production by recombinant microorganisms

Non-Patent Citations (5)

* Cited by examiner, † Cited by third party
Title
ATSUMI SHOTA ET AL: "Non-fermentative pathways for synthesis of branched-chain higher alcohols as biofuels", NATURE, vol. 451, no. 7174, 1 January 2008 (2008-01-01), pages 86 - 90, XP002491157, ISSN: 0028-0836 *
GIRBAL L ET AL: "Regulation of solvent production in Clostridium acetobutylicum", TRENDS IN BIOTECHNOLOGY, vol. 16, no. 1, 1 January 1998 (1998-01-01), pages 11 - 16, XP004101891, ISSN: 0167-7799 *
JONES D T ET AL: "Acetone-butanol fermentation revisited", MICROBIOLOGICAL REVIEWS, vol. 50, no. 4, 1 December 1986 (1986-12-01), pages 484 - 524, XP002425366, ISSN: 0146-0749 *
RATNATILLEKE ANANDA ET AL: "Cloning and sequencing of the coenzyme B12-binding domain of isobutyryl-CoA mutase from Streptomyces cinnamonensis, reconstitution of mutase activity, and characterization of the recombinant enzyme produced in Escherichia coli", JOURNAL OF BIOLOGICAL CHEMISTRY, vol. 274, no. 44, 29 October 1999 (1999-10-29), pages 31679 - 31685, XP002460830, ISSN: 0021-9258 *
WOODS D R: "The genetic engineering of microbial solvent production", TRENDS IN BIOTECHNOLOGY, vol. 13, no. 7, 1 July 1995 (1995-07-01), pages 259 - 264, XP004207180, ISSN: 0167-7799 *

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