WO2003045556A2 - Method, apparatus and article for microfluidic control via electrowetting, for chemical, biochemical and biological assays and the like - Google Patents
Method, apparatus and article for microfluidic control via electrowetting, for chemical, biochemical and biological assays and the like Download PDFInfo
- Publication number
- WO2003045556A2 WO2003045556A2 PCT/US2002/038047 US0238047W WO03045556A2 WO 2003045556 A2 WO2003045556 A2 WO 2003045556A2 US 0238047 W US0238047 W US 0238047W WO 03045556 A2 WO03045556 A2 WO 03045556A2
- Authority
- WO
- WIPO (PCT)
- Prior art keywords
- microfiuidic
- drive electrodes
- drive
- array
- fluid
- Prior art date
Links
Classifications
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
- B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
- B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
- B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
- B01L3/50273—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip characterised by the means or forces applied to move the fluids
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
- B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
- B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
- B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
- B01L3/502769—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip characterised by multiphase flow arrangements
- B01L3/502784—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip characterised by multiphase flow arrangements specially adapted for droplet or plug flow, e.g. digital microfluidics
- B01L3/502792—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip characterised by multiphase flow arrangements specially adapted for droplet or plug flow, e.g. digital microfluidics for moving individual droplets on a plate, e.g. by locally altering surface tension
-
- F—MECHANICAL ENGINEERING; LIGHTING; HEATING; WEAPONS; BLASTING
- F04—POSITIVE - DISPLACEMENT MACHINES FOR LIQUIDS; PUMPS FOR LIQUIDS OR ELASTIC FLUIDS
- F04B—POSITIVE-DISPLACEMENT MACHINES FOR LIQUIDS; PUMPS
- F04B17/00—Pumps characterised by combination with, or adaptation to, specific driving engines or motors
-
- F—MECHANICAL ENGINEERING; LIGHTING; HEATING; WEAPONS; BLASTING
- F04—POSITIVE - DISPLACEMENT MACHINES FOR LIQUIDS; PUMPS FOR LIQUIDS OR ELASTIC FLUIDS
- F04B—POSITIVE-DISPLACEMENT MACHINES FOR LIQUIDS; PUMPS
- F04B19/00—Machines or pumps having pertinent characteristics not provided for in, or of interest apart from, groups F04B1/00 - F04B17/00
- F04B19/006—Micropumps
-
- G—PHYSICS
- G02—OPTICS
- G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
- G02B26/00—Optical devices or arrangements for the control of light using movable or deformable optical elements
- G02B26/004—Optical devices or arrangements for the control of light using movable or deformable optical elements based on a displacement or a deformation of a fluid
- G02B26/005—Optical devices or arrangements for the control of light using movable or deformable optical elements based on a displacement or a deformation of a fluid based on electrowetting
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
- B01L2200/06—Fluid handling related problems
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2300/00—Additional constructional details
- B01L2300/08—Geometry, shape and general structure
- B01L2300/0809—Geometry, shape and general structure rectangular shaped
- B01L2300/0819—Microarrays; Biochips
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2300/00—Additional constructional details
- B01L2300/08—Geometry, shape and general structure
- B01L2300/089—Virtual walls for guiding liquids
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2400/00—Moving or stopping fluids
- B01L2400/04—Moving fluids with specific forces or mechanical means
- B01L2400/0403—Moving fluids with specific forces or mechanical means specific forces
- B01L2400/0415—Moving fluids with specific forces or mechanical means specific forces electrical forces, e.g. electrokinetic
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2400/00—Moving or stopping fluids
- B01L2400/04—Moving fluids with specific forces or mechanical means
- B01L2400/0403—Moving fluids with specific forces or mechanical means specific forces
- B01L2400/0415—Moving fluids with specific forces or mechanical means specific forces electrical forces, e.g. electrokinetic
- B01L2400/0427—Electrowetting
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
- B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
- B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
- B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
- B01L3/502715—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip characterised by interfacing components, e.g. fluidic, electrical, optical or mechanical interfaces
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
- B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
- B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
- B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
- B01L3/502738—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip characterised by integrated valves
Definitions
- Figure 9 is a schematic view of the microfiuidic system illustrating one exemplary embodiment a feedback subsystem employing a set of visual sensors.
- the system memory 36 of the computing system 14 can also include additional communications or networking software (not shown) for wired and/or wireless communications on networks, such as LAN 70, WAN or the Internet 72.
- the computing system 14 can include a Web client or browser 76 for communicating across the World Wide Web portion of the Internet 72 using standard protocol (e.g., Transmission Control Protocol/Internet Protocol (TCP/IP), User Datagram Protocol (UDP)).
- standard protocol e.g., Transmission Control Protocol/Internet Protocol (TCP/IP), User Datagram Protocol (UDP)
- TCP/IP Transmission Control Protocol/Internet Protocol
- UDP User Datagram Protocol
- a number of Web browsers are commercially available, such as NETSCAPE NAVIGATOR from America Online, and INTERNET EXPLORER available from Microsoft of Redmond, Washington.
- the microfiuidic platform 10 includes an interface 84 for providing communications between the computing system 14 and the various subsystems of the microfiuidic platform such as a feedback subsystem 86, row driver 28 and column driver 30.
- the microfiuidic platform also includes one or more voltage sources 88 for providing a potential to the drive electrodes 26 and/or ground electrode 32 in accordance with drive instructions supplied to the row and column drivers 28, 30 by the computing system 14. While shown as part of the microfiuidic structure 12, in some embodiments the voltage source 88 may be a discrete component, electrically couplable to the microfiuidic platform 10 and/or microfiuidic structure 12.
- the microfiuidic structure 12 may also contain an immiscible fluid 121 , for example air or some other immiscible fluid.
- the microfiuidic structure 12 may also incorporate humidity control since small bodies of fluids (i.e., droplets) 22a, 22b will rapidly evaporate if conditions near saturation are not used. Alternatively, or additionally, rather than carefully controlling humidity, another fluid 121 may be used in lieu of air to prevent evaporation.
- the row and column detection circuitry 140, 142 receive electrical signals from the position detection sensors 138 and provide position information 144 to the computing system 14, identifying the position of one or more bodies of fluid 22a, 22b in the microfiuidic structure 12.
- Suitable row and column detection circuitry 140, 142 is disclosed in U.S. Patent No. 5,194,862 issued March 16, 1993 to Edwards.
- Suitable processing software e.g. application programs 78
Abstract
Description
Claims
Priority Applications (4)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CA2472029A CA2472029C (en) | 2001-11-26 | 2002-11-26 | Method, apparatus and article for microfluidic control via electrowetting, for chemical, biochemical and biological assays and the like |
EP02794050A EP1450956A2 (en) | 2001-11-26 | 2002-11-26 | Method, apparatus and article for microfluidic control via electrowetting, for chemical, biochemical and biological assays and the like |
JP2003547050A JP2005510347A (en) | 2001-11-26 | 2002-11-26 | Methods, devices, and objects for microfluidic control via electrowetting for chemical, biochemical, biological assays, etc. |
AU2002359508A AU2002359508A1 (en) | 2001-11-26 | 2002-11-26 | Method, apparatus and article for microfluidic control via electrowetting, for chemical, biochemical and biological assays and the like |
Applications Claiming Priority (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US33362101P | 2001-11-26 | 2001-11-26 | |
US60/333,621 | 2001-11-26 |
Publications (2)
Publication Number | Publication Date |
---|---|
WO2003045556A2 true WO2003045556A2 (en) | 2003-06-05 |
WO2003045556A3 WO2003045556A3 (en) | 2004-04-15 |
Family
ID=23303561
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
PCT/US2002/038047 WO2003045556A2 (en) | 2001-11-26 | 2002-11-26 | Method, apparatus and article for microfluidic control via electrowetting, for chemical, biochemical and biological assays and the like |
Country Status (6)
Country | Link |
---|---|
US (1) | US7163612B2 (en) |
EP (1) | EP1450956A2 (en) |
JP (1) | JP2005510347A (en) |
AU (1) | AU2002359508A1 (en) |
CA (1) | CA2472029C (en) |
WO (1) | WO2003045556A2 (en) |
Cited By (64)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
FR2866493A1 (en) * | 2004-02-16 | 2005-08-19 | Commissariat Energie Atomique | DEVICE FOR CONTROLLING THE DISPLACEMENT OF A DROP BETWEEN TWO OR MORE SOLID SUBSTRATES |
FR2867268A1 (en) * | 2004-07-01 | 2005-09-09 | Commissariat Energie Atomique | Determining at least one geometric property of a drop of liquid by observing it on a design of known dimensions, for control of chemical and biological microfluids |
FR2871150A1 (en) * | 2004-06-04 | 2005-12-09 | Univ Lille Sciences Tech | DROP HANDLING DEVICE FOR BIOCHEMICAL ANALYSIS, DEVICE MANUFACTURING METHOD, AND MICROFLUIDIC ANALYSIS SYSTEM |
FR2871076A1 (en) * | 2004-06-04 | 2005-12-09 | Univ Lille Sciences Tech | DEVICE FOR LASER RADIATION DESORPTION INCORPORATING HANDLING OF THE LIQUID SAMPLE IN THE FORM OF INDIVIDUAL DROPS ENABLING THEIR CHEMICAL AND BIOCHEMICAL TREATMENT |
EP1627685A1 (en) * | 2004-08-17 | 2006-02-22 | Hitachi High-Technologies Corporation | Microfuidic-chip for chemical analysis based on electrowetting |
WO2006054198A1 (en) * | 2004-11-20 | 2006-05-26 | Koninklijke Philips Electronics N.V. | Controllable optical component |
JP2006300946A (en) * | 2005-04-19 | 2006-11-02 | Commissariat A L'energie Atomique | System for transferring method and mass of micro fluid between two types of immiscible phases |
WO2006138543A1 (en) * | 2005-06-16 | 2006-12-28 | Core-Microsolutions, Inc. | Biosensor detection by means of droplet driving, agitation, and evaporation |
WO2007010367A2 (en) * | 2005-07-19 | 2007-01-25 | Silicon Biosystems S.P.A. | Method and apparatus for the manipulation and/or the detection of particles |
WO2007022311A2 (en) * | 2005-08-17 | 2007-02-22 | The University Of Cincinnati | Electrofluidic textiles and cleaning implements using such electrofluidic textiles |
WO2008051310A2 (en) | 2006-05-09 | 2008-05-02 | Advanced Liquid Logic, Inc. | Droplet manipulation systems |
WO2008075053A2 (en) * | 2006-12-19 | 2008-06-26 | Cambridge Enterprise Limited | Particle transport and characterisation |
WO2008117210A1 (en) * | 2007-03-23 | 2008-10-02 | Koninklijke Philips Electronics N.V. | Integrated microfluidic device with local temperature control |
WO2009019658A2 (en) * | 2007-08-09 | 2009-02-12 | Koninklijke Philips Electronics N.V. | Integrated microfluidic device with local temperature control |
EP2053396A1 (en) * | 2007-10-22 | 2009-04-29 | Koninklijke Philips Electronics N.V. | Device and method for the monitoring of the movement of cells |
WO2009101584A2 (en) * | 2008-02-15 | 2009-08-20 | Koninklijke Philips Electronics N.V. | Driver system for lab-on-a-chip cartridges |
EP2250249A1 (en) * | 2008-02-11 | 2010-11-17 | Aaron R. Wheeler | Cell culture and cell assays using digital microfluidics |
US7842235B2 (en) | 2004-02-14 | 2010-11-30 | Roche Diagnostics Operations, Inc. | Test element, system, and method of controlling the wetting of same |
EP2404675A1 (en) * | 2010-07-06 | 2012-01-11 | Sharp Kabushiki Kaisha | Array element circuit and active matrix device |
US20120007608A1 (en) * | 2010-07-06 | 2012-01-12 | Sharp Kabushiki Kaisha | Array element circuit and active matrix device |
WO2012084707A1 (en) * | 2010-12-20 | 2012-06-28 | Technische Universität Ilmenau | Micropump for generating a fluid flow, pump system, and microchannel system |
US8389297B2 (en) | 2006-04-18 | 2013-03-05 | Duke University | Droplet-based affinity assay device and system |
US8388823B2 (en) | 2004-07-07 | 2013-03-05 | Silicon Biosystems S.P.A. | Method and apparatus for the separation and quantification of particles |
US8492168B2 (en) | 2006-04-18 | 2013-07-23 | Advanced Liquid Logic Inc. | Droplet-based affinity assays |
WO2013137499A1 (en) * | 2012-03-14 | 2013-09-19 | 엘지전자 주식회사 | Biosensor |
US8637317B2 (en) | 2006-04-18 | 2014-01-28 | Advanced Liquid Logic, Inc. | Method of washing beads |
US8637324B2 (en) | 2006-04-18 | 2014-01-28 | Advanced Liquid Logic, Inc. | Bead incubation and washing on a droplet actuator |
US8654571B2 (en) | 2010-07-06 | 2014-02-18 | Sharp Kabushiki Kaisha | Static random-access cell, active matrix device and array element circuit |
US8679856B2 (en) | 2006-03-27 | 2014-03-25 | Silicon Biosystems S.P.A. | Method and apparatus for the processing and/or analysis and/or selection of particles, in particular biological particles |
US8679315B2 (en) | 2005-10-26 | 2014-03-25 | Silicon Biosystems S.P.A. | Method and apparatus for characterizing and counting particles, in particular, biological particles |
EP2759342A3 (en) * | 2013-01-23 | 2014-08-13 | Sharp Kabushiki Kaisha | AM-EWOD device and method of driving with AC voltage |
US8809068B2 (en) | 2006-04-18 | 2014-08-19 | Advanced Liquid Logic, Inc. | Manipulation of beads in droplets and methods for manipulating droplets |
US8845872B2 (en) | 2006-04-18 | 2014-09-30 | Advanced Liquid Logic, Inc. | Sample processing droplet actuator, system and method |
US9039973B2 (en) | 2008-10-10 | 2015-05-26 | The Governing Council Of The University Of Toronto | Hybrid digital and channel microfluidic devices and methods of use thereof |
US9046514B2 (en) | 2007-02-09 | 2015-06-02 | Advanced Liquid Logic, Inc. | Droplet actuator devices and methods employing magnetic beads |
US9050606B2 (en) | 2006-04-13 | 2015-06-09 | Advanced Liquid Logic, Inc. | Bead manipulation techniques |
US9097662B2 (en) | 2006-04-18 | 2015-08-04 | Advanced Liquid Logic, Inc. | Droplet-based particle sorting |
US9192943B2 (en) | 2009-03-17 | 2015-11-24 | Silicon Biosystems S.P.A. | Microfluidic device for isolation of cells |
US9310287B2 (en) | 2007-10-29 | 2016-04-12 | Silicon Biosystems S.P.A. | Method and apparatus for the identification and handling of particles |
EP2867645A4 (en) * | 2012-06-27 | 2016-06-29 | Advanced Liquid Logic Inc | Techniques and droplet actuator designs for reducing bubble formation |
US9476856B2 (en) | 2006-04-13 | 2016-10-25 | Advanced Liquid Logic, Inc. | Droplet-based affinity assays |
US9945738B2 (en) | 2013-03-15 | 2018-04-17 | Genmark Diagnostics, Inc. | Devices and methods for monitoring and controlling temperature in a microfluidic environment |
US9950322B2 (en) | 2010-12-22 | 2018-04-24 | Menarini Silicon Biosystems S.P.A. | Microfluidic device for the manipulation of particles |
US10078078B2 (en) | 2006-04-18 | 2018-09-18 | Advanced Liquid Logic, Inc. | Bead incubation and washing on a droplet actuator |
US10232374B2 (en) | 2010-05-05 | 2019-03-19 | Miroculus Inc. | Method of processing dried samples using digital microfluidic device |
US10234447B2 (en) | 2008-11-04 | 2019-03-19 | Menarini Silicon Biosystems S.P.A. | Method for identification, selection and analysis of tumour cells |
US10376878B2 (en) | 2011-12-28 | 2019-08-13 | Menarini Silicon Biosystems S.P.A. | Devices, apparatus, kit and method for treating a biological sample |
US10408788B2 (en) | 2017-07-12 | 2019-09-10 | Sharp Life Science (Eu) Limited | Spacer for side loaded EWOD device |
US10464067B2 (en) | 2015-06-05 | 2019-11-05 | Miroculus Inc. | Air-matrix digital microfluidics apparatuses and methods for limiting evaporation and surface fouling |
EP3623049A1 (en) * | 2018-09-12 | 2020-03-18 | Sharp Life Science (EU) Limited | Microfluidic device and a method of loading fluid therein |
US10596572B2 (en) | 2016-08-22 | 2020-03-24 | Miroculus Inc. | Feedback system for parallel droplet control in a digital microfluidic device |
US10695762B2 (en) | 2015-06-05 | 2020-06-30 | Miroculus Inc. | Evaporation management in digital microfluidic devices |
US10895575B2 (en) | 2008-11-04 | 2021-01-19 | Menarini Silicon Biosystems S.P.A. | Method for identification, selection and analysis of tumour cells |
US11255809B2 (en) | 2006-04-18 | 2022-02-22 | Advanced Liquid Logic, Inc. | Droplet-based surface modification and washing |
US11253860B2 (en) | 2016-12-28 | 2022-02-22 | Miroculus Inc. | Digital microfluidic devices and methods |
US11311882B2 (en) | 2017-09-01 | 2022-04-26 | Miroculus Inc. | Digital microfluidics devices and methods of using them |
US11413617B2 (en) | 2017-07-24 | 2022-08-16 | Miroculus Inc. | Digital microfluidics systems and methods with integrated plasma collection device |
US11524298B2 (en) | 2019-07-25 | 2022-12-13 | Miroculus Inc. | Digital microfluidics devices and methods of use thereof |
US11577244B2 (en) | 2018-09-12 | 2023-02-14 | Sharp Life Science (Eu) Limited | Microfluidic device and a method of loading fluid therein |
FR3127810A1 (en) | 2021-10-01 | 2023-04-07 | Commissariat à l'Energie Atomique et aux Energies Alternatives | Drop sorting process |
US11623219B2 (en) | 2017-04-04 | 2023-04-11 | Miroculus Inc. | Digital microfluidics apparatuses and methods for manipulating and processing encapsulated droplets |
US11738345B2 (en) | 2019-04-08 | 2023-08-29 | Miroculus Inc. | Multi-cartridge digital microfluidics apparatuses and methods of use |
US11772093B2 (en) | 2022-01-12 | 2023-10-03 | Miroculus Inc. | Methods of mechanical microfluidic manipulation |
US11921028B2 (en) | 2011-10-28 | 2024-03-05 | Menarini Silicon Biosystems S.P.A. | Method and device for optical analysis of particles at low temperatures |
Families Citing this family (218)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
AU2001280796A1 (en) * | 2000-07-25 | 2002-02-05 | The Regents Of The University Of California | Electrowetting-driven micropumping |
NL1019613C2 (en) * | 2001-12-19 | 2003-06-20 | Micronit Microfluidics Bv | Method for dividing a substrate into a number of individual chip parts. |
US6911132B2 (en) * | 2002-09-24 | 2005-06-28 | Duke University | Apparatus for manipulating droplets by electrowetting-based techniques |
US7329545B2 (en) | 2002-09-24 | 2008-02-12 | Duke University | Methods for sampling a liquid flow |
US6989234B2 (en) * | 2002-09-24 | 2006-01-24 | Duke University | Method and apparatus for non-contact electrostatic actuation of droplets |
US6747777B1 (en) * | 2003-02-24 | 2004-06-08 | Cymscape Incorporated | Reflective microfluidics display particularly suited for large format applications |
JP4256270B2 (en) * | 2004-01-19 | 2009-04-22 | 国立大学法人 筑波大学 | Liquid feeding device and driving method thereof |
WO2005118129A1 (en) * | 2004-05-27 | 2005-12-15 | Stratos Biosystems, Llc | Solid-phase affinity-based method for preparing and manipulating an analyte-containing solution |
EP1765501A1 (en) | 2004-05-28 | 2007-03-28 | Board of Regents, The University of Texas System | Programmable fluidic processors |
US7121998B1 (en) * | 2004-06-08 | 2006-10-17 | Eurica Califorrniaa | Vented microcradle for prenidial incubator |
US7878064B2 (en) * | 2004-06-12 | 2011-02-01 | Akubio Limited | Analytical apparatus with array of sensors and calibrating element |
EP1761819A1 (en) * | 2004-06-23 | 2007-03-14 | Koninklijke Philips Electronics N.V. | Bi-stable electrowetting optical element and driving method therefore |
US7693666B2 (en) * | 2004-07-07 | 2010-04-06 | Rensselaer Polytechnic Institute | Method, system, and program product for controlling chemical reactions in a digital microfluidic system |
WO2006026351A1 (en) | 2004-08-26 | 2006-03-09 | Applera Corporation | Electrowetting dispensing devices and related methods |
US7515010B2 (en) * | 2004-10-08 | 2009-04-07 | The Regents Of The University Of California | Nanoscale relaxation oscillator |
WO2006044966A1 (en) * | 2004-10-18 | 2006-04-27 | Stratos Biosystems, Llc | Single-sided apparatus for manipulating droplets by electrowetting-on-dielectric techniques |
US20060132927A1 (en) * | 2004-11-30 | 2006-06-22 | Yoon Frank C | Electrowetting chromatophore |
US20060153745A1 (en) * | 2005-01-11 | 2006-07-13 | Applera Corporation | Fluid processing device for oligonucleotide synthesis and analysis |
JP4427461B2 (en) * | 2005-01-21 | 2010-03-10 | 株式会社日立ハイテクノロジーズ | Chemical analysis apparatus and analysis device |
PL1859330T3 (en) * | 2005-01-28 | 2013-01-31 | Univ Duke | Apparatuses and methods for manipulating droplets on a printed circuit board |
US7454988B2 (en) * | 2005-02-10 | 2008-11-25 | Applera Corporation | Method for fluid sampling using electrically controlled droplets |
JP4632300B2 (en) * | 2005-02-14 | 2011-02-16 | 国立大学法人 筑波大学 | Liquid feeding device |
JP4253028B2 (en) * | 2005-04-12 | 2009-04-08 | 古河電気工業株式会社 | Liquid actuator |
JP2008539759A (en) | 2005-05-11 | 2008-11-20 | ナノリティックス・インコーポレイテッド | Method and apparatus for performing biochemical or chemical reactions at multiple temperatures |
US8092664B2 (en) | 2005-05-13 | 2012-01-10 | Applied Biosystems Llc | Electrowetting-based valving and pumping systems |
JP4268955B2 (en) * | 2005-05-30 | 2009-05-27 | 株式会社日立ハイテクノロジーズ | Chemical analyzer |
KR101129435B1 (en) * | 2005-06-15 | 2012-03-27 | 삼성전자주식회사 | Electrowetting display panel and manufacturing method thereof |
US20070095407A1 (en) * | 2005-10-28 | 2007-05-03 | Academia Sinica | Electrically controlled addressable multi-dimensional microfluidic device and method |
JP4713306B2 (en) * | 2005-11-09 | 2011-06-29 | 株式会社日立製作所 | Liquid transfer device |
US7619609B2 (en) * | 2005-12-28 | 2009-11-17 | Palo Alto Research Center Incorporated | Fluidic display apparatus |
JP4910805B2 (en) * | 2006-03-31 | 2012-04-04 | ブラザー工業株式会社 | Droplet transfer device, valve, storage device, and display device |
US8613889B2 (en) * | 2006-04-13 | 2013-12-24 | Advanced Liquid Logic, Inc. | Droplet-based washing |
US8980198B2 (en) * | 2006-04-18 | 2015-03-17 | Advanced Liquid Logic, Inc. | Filler fluids for droplet operations |
US8927296B2 (en) * | 2006-04-18 | 2015-01-06 | Advanced Liquid Logic, Inc. | Method of reducing liquid volume surrounding beads |
US7763471B2 (en) * | 2006-04-18 | 2010-07-27 | Advanced Liquid Logic, Inc. | Method of electrowetting droplet operations for protein crystallization |
US7815871B2 (en) * | 2006-04-18 | 2010-10-19 | Advanced Liquid Logic, Inc. | Droplet microactuator system |
US8658111B2 (en) | 2006-04-18 | 2014-02-25 | Advanced Liquid Logic, Inc. | Droplet actuators, modified fluids and methods |
US7816121B2 (en) * | 2006-04-18 | 2010-10-19 | Advanced Liquid Logic, Inc. | Droplet actuation system and method |
US8470606B2 (en) * | 2006-04-18 | 2013-06-25 | Duke University | Manipulation of beads in droplets and methods for splitting droplets |
US8716015B2 (en) | 2006-04-18 | 2014-05-06 | Advanced Liquid Logic, Inc. | Manipulation of cells on a droplet actuator |
US7939021B2 (en) * | 2007-05-09 | 2011-05-10 | Advanced Liquid Logic, Inc. | Droplet actuator analyzer with cartridge |
US8041463B2 (en) * | 2006-05-09 | 2011-10-18 | Advanced Liquid Logic, Inc. | Modular droplet actuator drive |
WO2009111769A2 (en) | 2008-03-07 | 2009-09-11 | Advanced Liquid Logic, Inc. | Reagent and sample preparation and loading on a fluidic device |
US7822510B2 (en) * | 2006-05-09 | 2010-10-26 | Advanced Liquid Logic, Inc. | Systems, methods, and products for graphically illustrating and controlling a droplet actuator |
WO2007133714A2 (en) * | 2006-05-12 | 2007-11-22 | Stratos Biosystems, Llc | Analyte focusing biochips for affinity mass spectrometry |
JP4792338B2 (en) * | 2006-07-04 | 2011-10-12 | 株式会社日立製作所 | Liquid transfer device |
JP4651715B2 (en) * | 2006-07-05 | 2011-03-16 | 株式会社日立ハイテクノロジーズ | Liquid analyzer |
US20080121045A1 (en) * | 2006-11-29 | 2008-05-29 | Cole Matthew C | Multiplexed sensor array |
WO2008091848A2 (en) * | 2007-01-22 | 2008-07-31 | Advanced Liquid Logic, Inc. | Surface assisted fluid loading and droplet dispensing |
EP2109774B1 (en) | 2007-02-15 | 2018-07-04 | Advanced Liquid Logic, Inc. | Capacitance detection in a droplet actuator |
US20080199861A1 (en) * | 2007-02-15 | 2008-08-21 | Honeywell International, Inc. | Real-time microarray apparatus and methods related thereto |
US20100025250A1 (en) * | 2007-03-01 | 2010-02-04 | Advanced Liquid Logic, Inc. | Droplet Actuator Structures |
US7791815B2 (en) * | 2007-03-13 | 2010-09-07 | Varioptic S.A. | Dielectric coatings for electrowetting applications |
US8440392B2 (en) | 2007-03-22 | 2013-05-14 | Advanced Liquid Logic Inc. | Method of conducting a droplet based enzymatic assay |
WO2011084703A2 (en) | 2009-12-21 | 2011-07-14 | Advanced Liquid Logic, Inc. | Enzyme assays on a droplet actuator |
US8037903B2 (en) * | 2007-04-04 | 2011-10-18 | Micropoint Bioscience, Inc. | Micromachined electrowetting microfluidic valve |
CN101743304B (en) * | 2007-04-10 | 2013-04-24 | 先进流体逻辑公司 | Droplet dispensing device and methods |
KR101471054B1 (en) * | 2007-05-24 | 2014-12-09 | 디지털 바이오시스템즈 | Electrowetting based digital microfluidics |
WO2009002920A1 (en) | 2007-06-22 | 2008-12-31 | Advanced Liquid Logic, Inc. | Droplet-based nucleic acid amplification in a temperature gradient |
WO2009003184A1 (en) | 2007-06-27 | 2008-12-31 | Digital Biosystems | Digital microfluidics based apparatus for heat-exchanging chemical processes |
WO2009021233A2 (en) | 2007-08-09 | 2009-02-12 | Advanced Liquid Logic, Inc. | Pcb droplet actuator fabrication |
US8591830B2 (en) * | 2007-08-24 | 2013-11-26 | Advanced Liquid Logic, Inc. | Bead manipulations on a droplet actuator |
WO2009032863A2 (en) * | 2007-09-04 | 2009-03-12 | Advanced Liquid Logic, Inc. | Droplet actuator with improved top substrate |
US20090086064A1 (en) * | 2007-09-27 | 2009-04-02 | Micron Technology, Inc. | Dynamic adaptive color filter array |
DE102007047415B3 (en) * | 2007-10-04 | 2009-04-02 | Dräger Medical AG & Co. KG | A liquid vaporizer |
WO2009052123A2 (en) * | 2007-10-17 | 2009-04-23 | Advanced Liquid Logic, Inc. | Multiplexed detection schemes for a droplet actuator |
US8460528B2 (en) * | 2007-10-17 | 2013-06-11 | Advanced Liquid Logic Inc. | Reagent storage and reconstitution for a droplet actuator |
US20100236929A1 (en) * | 2007-10-18 | 2010-09-23 | Advanced Liquid Logic, Inc. | Droplet Actuators, Systems and Methods |
US8562807B2 (en) * | 2007-12-10 | 2013-10-22 | Advanced Liquid Logic Inc. | Droplet actuator configurations and methods |
MX2010007034A (en) | 2007-12-23 | 2010-09-14 | Advanced Liquid Logic Inc | Droplet actuator configurations and methods of conducting droplet operations. |
US8852952B2 (en) | 2008-05-03 | 2014-10-07 | Advanced Liquid Logic, Inc. | Method of loading a droplet actuator |
US20110097763A1 (en) * | 2008-05-13 | 2011-04-28 | Advanced Liquid Logic, Inc. | Thermal Cycling Method |
CN101614729B (en) * | 2008-06-27 | 2013-04-24 | 博奥生物有限公司 | Microelectrode array device and special device for cell manipulation and electrophysiological signal detection |
DE102008038457B4 (en) * | 2008-08-20 | 2013-10-24 | Advanced Display Technology Ag | Device for fluidic display |
TWI418848B (en) * | 2008-08-29 | 2013-12-11 | Prime View Int Co Ltd | Pixel structure for use in a display and display using the same |
US8187864B2 (en) | 2008-10-01 | 2012-05-29 | The Governing Council Of The University Of Toronto | Exchangeable sheets pre-loaded with reagent depots for digital microfluidics |
US8053239B2 (en) | 2008-10-08 | 2011-11-08 | The Governing Council Of The University Of Toronto | Digital microfluidic method for protein extraction by precipitation from heterogeneous mixtures |
US8877512B2 (en) * | 2009-01-23 | 2014-11-04 | Advanced Liquid Logic, Inc. | Bubble formation techniques using physical or chemical features to retain a gas bubble within a droplet actuator |
US8202736B2 (en) * | 2009-02-26 | 2012-06-19 | The Governing Council Of The University Of Toronto | Method of hormone extraction using digital microfluidics |
US9851365B2 (en) | 2009-02-26 | 2017-12-26 | The Governing Council Of The University Of Toronto | Digital microfluidic liquid-liquid extraction device and method of use thereof |
TW201102734A (en) * | 2009-07-07 | 2011-01-16 | Ind Tech Res Inst | Smart display devices |
US8926065B2 (en) | 2009-08-14 | 2015-01-06 | Advanced Liquid Logic, Inc. | Droplet actuator devices and methods |
JP5610258B2 (en) * | 2009-09-09 | 2014-10-22 | 国立大学法人 筑波大学 | Liquid feeding device |
US8846414B2 (en) | 2009-09-29 | 2014-09-30 | Advanced Liquid Logic, Inc. | Detection of cardiac markers on a droplet actuator |
US9005544B2 (en) * | 2009-10-15 | 2015-04-14 | The Regents Of The University Of California | Digital microfluidic platform for radiochemistry |
WO2011057197A2 (en) | 2009-11-06 | 2011-05-12 | Advanced Liquid Logic, Inc. | Integrated droplet actuator for gel electrophoresis and molecular analysis |
AU2011221243B2 (en) | 2010-02-25 | 2016-06-02 | Advanced Liquid Logic, Inc. | Method of making nucleic acid libraries |
US9248450B2 (en) | 2010-03-30 | 2016-02-02 | Advanced Liquid Logic, Inc. | Droplet operations platform |
US20190300945A1 (en) | 2010-04-05 | 2019-10-03 | Prognosys Biosciences, Inc. | Spatially Encoded Biological Assays |
PL2556171T3 (en) | 2010-04-05 | 2016-04-29 | Prognosys Biosciences Inc | Spatially encoded biological assays |
US10787701B2 (en) | 2010-04-05 | 2020-09-29 | Prognosys Biosciences, Inc. | Spatially encoded biological assays |
US8419273B2 (en) | 2010-05-03 | 2013-04-16 | Sharp Kabushiki Kaisha | Array element for temperature sensor array circuit, temperature sensor array circuit utilizing such array element, and AM-EWOD device including such a temperature sensor array circuit |
US9011662B2 (en) | 2010-06-30 | 2015-04-21 | Advanced Liquid Logic, Inc. | Droplet actuator assemblies and methods of making same |
US9476811B2 (en) | 2010-10-01 | 2016-10-25 | The Governing Council Of The University Of Toronto | Digital microfluidic devices and methods incorporating a solid phase |
WO2012068055A2 (en) | 2010-11-17 | 2012-05-24 | Advanced Liquid Logic, Inc. | Capacitance detection in a droplet actuator |
TW201224507A (en) | 2010-12-13 | 2012-06-16 | Ind Tech Res Inst | Electrofluidic device and operation method thereof |
US8173000B1 (en) | 2011-01-18 | 2012-05-08 | Sharp Kabushiki Kaisha | Active matrix device and method of driving the same |
US8828336B2 (en) * | 2011-02-02 | 2014-09-09 | Sharp Kabushiki Kaisha | Active matrix device |
GB201106254D0 (en) | 2011-04-13 | 2011-05-25 | Frisen Jonas | Method and product |
US8339711B2 (en) | 2011-04-22 | 2012-12-25 | Sharp Kabushiki Kaisha | Active matrix device and method of driving the same |
CN103562729A (en) | 2011-05-02 | 2014-02-05 | 先进流体逻辑公司 | Molecular diagnostics platform |
EP2707131B1 (en) | 2011-05-09 | 2019-04-24 | Advanced Liquid Logic, Inc. | Microfluidic feedback using impedance detection |
WO2012154794A2 (en) | 2011-05-10 | 2012-11-15 | Advanced Liquid Logic, Inc. | Enzyme concentration and assays |
BR112014000257A2 (en) | 2011-07-06 | 2017-03-01 | Advanced Liquid Logic Inc | reagent storage in a drop actuator |
US8901043B2 (en) | 2011-07-06 | 2014-12-02 | Advanced Liquid Logic, Inc. | Systems for and methods of hybrid pyrosequencing |
WO2013009927A2 (en) | 2011-07-11 | 2013-01-17 | Advanced Liquid Logic, Inc. | Droplet actuators and techniques for droplet-based assays |
KR20130009504A (en) | 2011-07-15 | 2013-01-23 | 삼성전자주식회사 | Method and device for adjusting aperture |
WO2013016413A2 (en) | 2011-07-25 | 2013-01-31 | Advanced Liquid Logic Inc | Droplet actuator apparatus and system |
US8980075B2 (en) | 2011-07-29 | 2015-03-17 | The Texas A & M University System | Digital microfluidic platform for actuating and heating individual liquid droplets |
US20130062205A1 (en) | 2011-09-14 | 2013-03-14 | Sharp Kabushiki Kaisha | Active matrix device for fluid control by electro-wetting and dielectrophoresis and method of driving |
WO2013070627A2 (en) | 2011-11-07 | 2013-05-16 | Illumina, Inc. | Integrated sequencing apparatuses and methods of use |
WO2013078216A1 (en) | 2011-11-21 | 2013-05-30 | Advanced Liquid Logic Inc | Glucose-6-phosphate dehydrogenase assays |
JP5468687B2 (en) * | 2012-01-11 | 2014-04-09 | シャープ株式会社 | Static random access cell, matrix active matrix device, array element circuit |
KR101903789B1 (en) * | 2012-02-17 | 2018-10-02 | 리쿠아비스타 비.브이. | Eletrowetting display device and driving method thereof |
US8981789B2 (en) | 2012-04-16 | 2015-03-17 | Sharp Kabushiki Kaisha | Active matrix device and method of driving the same |
US9223317B2 (en) | 2012-06-14 | 2015-12-29 | Advanced Liquid Logic, Inc. | Droplet actuators that include molecular barrier coatings |
WO2014062551A1 (en) | 2012-10-15 | 2014-04-24 | Advanced Liquid Logic, Inc. | Digital microfluidics cartridge and system for operating a flow cell |
US20140322706A1 (en) | 2012-10-24 | 2014-10-30 | Jon Faiz Kayyem | Integrated multipelx target analysis |
EP2912432B1 (en) | 2012-10-24 | 2018-07-04 | Genmark Diagnostics Inc. | Integrated multiplex target analysis |
US9458543B2 (en) | 2012-12-04 | 2016-10-04 | Sharp Kabushiki Kaisha | Active matrix electrowetting-on-dielectric device |
US9239328B2 (en) | 2012-12-17 | 2016-01-19 | Taiwan Semiconductor Manufacturing Company, Ltd. | Systems and methods for an integrated bio-entity manipulation and processing semiconductor device |
US20140273045A1 (en) * | 2013-03-15 | 2014-09-18 | Lester F. Ludwig | Modular Biochemical Signaling Laboratory Breadboard for Disease Research, Drug Discovery, Cell Biology, and Other Applications |
WO2014150905A2 (en) | 2013-03-15 | 2014-09-25 | Genmark Diagnostics, Inc. | Systems, methods, and apparatus for manipulating deformable fluid vessels |
WO2014210225A1 (en) | 2013-06-25 | 2014-12-31 | Prognosys Biosciences, Inc. | Methods and systems for determining spatial patterns of biological targets in a sample |
US10124351B2 (en) | 2013-08-13 | 2018-11-13 | Advanced Liquid Logic, Inc. | Methods of improving accuracy and precision of droplet metering using an on-actuator reservoir as the fluid input |
EP3038834B1 (en) | 2013-08-30 | 2018-12-12 | Illumina, Inc. | Manipulation of droplets on hydrophilic or variegated-hydrophilic surfaces |
JP6107961B2 (en) * | 2013-10-17 | 2017-04-05 | 日産自動車株式会社 | Magnetic air conditioner |
US9498778B2 (en) | 2014-11-11 | 2016-11-22 | Genmark Diagnostics, Inc. | Instrument for processing cartridge for performing assays in a closed sample preparation and reaction system |
USD881409S1 (en) | 2013-10-24 | 2020-04-14 | Genmark Diagnostics, Inc. | Biochip cartridge |
TWI510780B (en) | 2014-03-20 | 2015-12-01 | Univ Nat Chiao Tung | An inspecting equipment and a biochip |
AU2015253299B2 (en) | 2014-04-29 | 2018-06-14 | Illumina, Inc. | Multiplexed single cell gene expression analysis using template switch and tagmentation |
JP2017515118A (en) | 2014-05-01 | 2017-06-08 | アリゾナ ボード オブ リージェンツ オン ビハーフ オブ アリゾナ ステート ユニバーシティ | Flexible optical biosensor for multiple pathogen detection |
US10118173B2 (en) | 2014-10-09 | 2018-11-06 | Illumina, Inc. | Method and device for separating immiscible liquids to effectively isolate at least one of the liquids |
US9598722B2 (en) | 2014-11-11 | 2017-03-21 | Genmark Diagnostics, Inc. | Cartridge for performing assays in a closed sample preparation and reaction system |
US10005080B2 (en) | 2014-11-11 | 2018-06-26 | Genmark Diagnostics, Inc. | Instrument and cartridge for performing assays in a closed sample preparation and reaction system employing electrowetting fluid manipulation |
US9659534B2 (en) * | 2014-12-29 | 2017-05-23 | Amazon Technologies, Inc. | Reducing visual artifacts and reducing power consumption in electrowetting displays |
ES2786652T3 (en) | 2015-02-10 | 2020-10-13 | Illumina Inc | Methods and compositions for analyzing cellular components |
US10576471B2 (en) | 2015-03-20 | 2020-03-03 | Illumina, Inc. | Fluidics cartridge for use in the vertical or substantially vertical position |
EP3901282B1 (en) | 2015-04-10 | 2023-06-28 | Spatial Transcriptomics AB | Spatially distinguished, multiplex nucleic acid analysis of biological specimens |
US9841402B2 (en) | 2015-04-15 | 2017-12-12 | Sharp Life Science (Eu) Limited | Multifunction electrode with combined heating and EWOD drive functionality |
CA3176115A1 (en) | 2015-04-22 | 2016-10-27 | Berkeley Lights, Inc. | Microfluidic device for culturing biological cells and methods of use thereof |
CN113624953A (en) | 2015-05-11 | 2021-11-09 | 亿明达股份有限公司 | Platform for discovery and analysis of therapeutic agents |
US10591474B2 (en) | 2015-06-03 | 2020-03-17 | Arizona Board Of Regents On Behalf Of Arizona State University | Point-of-care fluorescent immunoassay for identifying biomarkers in patient biofluid samples |
US9539573B1 (en) | 2015-06-23 | 2017-01-10 | Sharp Kabushiki Kaisha | EWOD device with calibrated serial dilution function |
WO2017007757A1 (en) | 2015-07-06 | 2017-01-12 | Illumina, Inc. | Balanced ac modulation for driving droplet operations electrodes |
CA2992597C (en) | 2015-08-14 | 2024-03-05 | Illumina, Inc. | Systems and methods using magnetically-responsive sensors for determining a genetic characteristic |
US11061015B2 (en) | 2015-08-28 | 2021-07-13 | Sharp Life Science (Eu) Limited | Droplet microfluidic device and methods of sensing the results of an assay therein |
AU2016316773B2 (en) | 2015-08-28 | 2020-01-30 | Illumina, Inc. | Nucleic acid sequence analysis from single cells |
WO2017037078A1 (en) | 2015-09-02 | 2017-03-09 | Illumina Cambridge Limited | Systems and methods of improving droplet operations in fluidic systems |
US10450598B2 (en) | 2015-09-11 | 2019-10-22 | Illumina, Inc. | Systems and methods for obtaining a droplet having a designated concentration of a substance-of-interest |
US10078986B2 (en) | 2015-09-15 | 2018-09-18 | Sharp Life Science (Eu) Limited | Active matrix device and method of driving |
US10113985B2 (en) | 2015-09-15 | 2018-10-30 | Sharp Life Science (Eu) Limited | Active matrix device and method of driving |
RU2719991C2 (en) | 2015-10-22 | 2020-04-23 | Иллюмина, Инк. | Filling fluid medium for jet devices |
US10799865B2 (en) | 2015-10-27 | 2020-10-13 | Berkeley Lights, Inc. | Microfluidic apparatus having an optimized electrowetting surface and related systems and methods |
EP3384046B1 (en) | 2015-12-01 | 2021-04-28 | Illumina, Inc. | Digital microfluidic system for single-cell isolation and characterization of analytes |
WO2017095845A1 (en) | 2015-12-01 | 2017-06-08 | Illumina, Inc. | Liquid storage and delivery mechanisms and methods |
EP3411815A4 (en) * | 2016-02-04 | 2019-08-28 | Hewlett-Packard Development Company, L.P. | Managing a microfluidics device |
ES2786974T3 (en) | 2016-04-07 | 2020-10-14 | Illumina Inc | Methods and systems for the construction of standard nucleic acid libraries |
AU2017271673B2 (en) | 2016-05-26 | 2022-04-14 | Berkeley Lights, Inc. | Covalently modified surfaces, kits, and methods of preparation and use |
CN109414663B (en) * | 2016-06-29 | 2022-05-17 | 数字生物系统公司 | Creating high resolution temperature profiles in digital microfluidic devices |
US20180059056A1 (en) | 2016-08-30 | 2018-03-01 | Sharp Life Science (Eu) Limited | Electrowetting on dielectric device including surfactant containing siloxane group |
EP3357578B1 (en) | 2017-02-06 | 2021-01-06 | Sharp Life Science (EU) Limited | Temperature control system for microfluidic device |
EP3357576B1 (en) | 2017-02-06 | 2019-10-16 | Sharp Life Science (EU) Limited | Microfluidic device with multiple temperature zones |
US10330919B2 (en) | 2017-03-31 | 2019-06-25 | Sharp Life Science (Eu) Limited | AM-EWOD device and control methods with intermittent actuation patterns |
US10330920B2 (en) | 2017-04-04 | 2019-06-25 | Sharp Life Science (Eu) Limited | Droplet actuation method for a microfluidic device |
US10850272B2 (en) | 2017-05-04 | 2020-12-01 | Massachusetts Institute Of Technology | Methods and apparatus for processing droplets |
US10695761B2 (en) | 2017-05-30 | 2020-06-30 | Sharp Life Science (Eu) Limited | Microfluidic device with multiple temperature zones and enhanced temperature control |
US10730048B2 (en) | 2017-06-21 | 2020-08-04 | Sharp Life Science (Eu) Limited | EWOD device with holdback feature for fluid loading |
US10994274B2 (en) | 2017-07-12 | 2021-05-04 | Sharp Life Science (Eu) Limited | Housing for simple assembly of an EWOD device |
US10315911B2 (en) | 2017-07-12 | 2019-06-11 | Sharp Life Science (Eu) Limited | Spacer for side loaded EWOD device |
US10926256B2 (en) | 2017-07-12 | 2021-02-23 | Sharp Life Science (Eu) Limited | Housing for simple assembly of an EWOD device |
US10369570B2 (en) | 2017-07-27 | 2019-08-06 | Sharp Life Science (Eu) Limited | Microfluidic device with droplet pre-charge on input |
CN107527595B (en) * | 2017-09-27 | 2019-06-07 | 京东方科技集团股份有限公司 | A kind of microfluidic system and its driving method |
CN107649223B (en) * | 2017-09-27 | 2019-10-15 | 京东方科技集团股份有限公司 | Drop control detection device and its working method |
CN115007233B (en) * | 2017-10-18 | 2023-11-10 | 核蛋白有限公司 | Digital microfluidic device including dual substrates with thin film transistors and capacitive sensing |
GB2569630B (en) | 2017-12-21 | 2022-10-12 | Sharp Life Science Eu Ltd | Droplet Interfaces in Electro-wetting Devices |
ES2937927T3 (en) | 2018-01-29 | 2023-04-03 | St Jude Childrens Res Hospital Inc | Method for nucleic acid amplification |
JP7333794B2 (en) | 2018-05-24 | 2023-08-25 | オックスフォード ナノポール テクノロジーズ ピーエルシー | Droplet interface in electrowetting devices |
US10814324B2 (en) | 2018-05-29 | 2020-10-27 | Sharp Life Science (Eu) Limited | AM-EWOD array element circuitry with shared sensor components |
US11198130B2 (en) | 2018-06-21 | 2021-12-14 | Sharp Life Science (Eu) Limited | EWOD system and methods to increase dynamic range for digital nucleic acid amplification |
US11207688B2 (en) | 2018-06-25 | 2021-12-28 | Sharp Life Science (Eu) Limited | Adpative droplet operations in an AM-EWOD device based on test measurement of droplet properties |
CN108844900B (en) * | 2018-07-02 | 2022-02-22 | 京东方科技集团股份有限公司 | Microfluidic detection device and method |
US10859813B2 (en) | 2018-07-03 | 2020-12-08 | Sharp Life Science (Eu) Limited | Optically black AM-EWOD array element structure |
WO2020026200A1 (en) * | 2018-08-02 | 2020-02-06 | Mgi Tech Co., Ltd. | Apparatus and method for forming droplets having predetermined volume by electrowetting |
US11207686B2 (en) | 2018-08-21 | 2021-12-28 | Sharp Life Science (Eu) Limited | Microfluidic device and methods for digital assays in biological analyses |
US11353759B2 (en) | 2018-09-17 | 2022-06-07 | Nuclera Nucleics Ltd. | Backplanes with hexagonal and triangular electrodes |
US10913067B2 (en) | 2018-10-01 | 2021-02-09 | Sharp Life Science (Eu) Limited | Barrier droplet configurations against migration between droplets on AM-EWOD devices |
US11511096B2 (en) | 2018-10-15 | 2022-11-29 | E Ink Corporation | Digital microfluidic delivery device |
CN109465041B (en) * | 2018-11-12 | 2021-04-27 | 京东方科技集团股份有限公司 | Microfluidic device, control method of microfluidic device, and micro total analysis system |
JP2022513138A (en) | 2018-11-28 | 2022-02-07 | オックスフォード ナノポール テクノロジーズ ピーエルシー | Sensing system and how it works |
US10997931B2 (en) | 2018-12-03 | 2021-05-04 | Sharp Life Science (Eu) Limited | AM-EWOD array element circuitry with high sensitivity for small capacitance |
US10981168B2 (en) | 2018-12-03 | 2021-04-20 | Sharp Life Science (Eu) Limited | AM-EWOD array element circuitry with integrated sensing and method of sensing droplet merging |
US10978007B2 (en) | 2018-12-03 | 2021-04-13 | Sharp Life Science (Eu) Limited | AM-EWOD circuit configuration with sensing column detection circuit |
US11117134B2 (en) | 2019-01-08 | 2021-09-14 | Sharp Life Science (Eu) Limited | Non-poissonian droplet partitioning using feedback |
CN111450906B (en) * | 2019-01-22 | 2022-02-25 | 北京纳米能源与系统研究所 | Self-driven electrowetting valve, paper-based microfluid chip and immunodetection device |
EP3924513B1 (en) | 2019-02-14 | 2023-04-12 | Pacific Biosciences of California, Inc. | Mitigating adverse impacts of detection systems on nucleic acids and other biological analytes |
US11219898B2 (en) | 2019-02-15 | 2022-01-11 | Sharp Life Science (Eu) Limited | Molecular separation by diffusion using an EWOD device |
US10870114B2 (en) | 2019-03-11 | 2020-12-22 | Sharp Life Science (Eu) Limited | EWOD cartridge position sensing when docked in EWOD instrument |
EP3983126A2 (en) | 2019-06-14 | 2022-04-20 | Sharp Life Science (EU) Limited | Magnetic particle extraction in an ewod instrument |
EP3756762A1 (en) | 2019-06-25 | 2020-12-30 | Sharp Life Science (EU) Limited | Microfluidic device and a method of manipulating droplets therein |
EP3756761A1 (en) | 2019-06-25 | 2020-12-30 | Sharp Life Science (EU) Limited | Microfluidic device and a method of manipulating droplets therein |
US11376597B2 (en) | 2019-07-08 | 2022-07-05 | Sharp Life Science (Eu) Limited | Use of multiple filler fluids in an EWOD device via the use of an electrowetting gate |
EP3812041A1 (en) | 2019-10-25 | 2021-04-28 | Sharp Life Science (EU) Limited | Method of operating ewod device with sensing apparatus |
EP3812042A1 (en) | 2019-10-25 | 2021-04-28 | Sharp Life Science (EU) Limited | Ewod device with sensing apparatus |
US11235325B2 (en) | 2019-11-11 | 2022-02-01 | Sharp Life Science (Eu) Limited | Microfluidic system including remote heat spreader |
US11927740B2 (en) | 2019-11-20 | 2024-03-12 | Nuclera Ltd | Spatially variable hydrophobic layers for digital microfluidics |
US11524297B2 (en) | 2019-12-03 | 2022-12-13 | Sharp Life Science (Eu) Limited | Method of concentrating particles in a liquid droplet using an EWOD device with sensing apparatus |
WO2021146573A1 (en) | 2020-01-17 | 2021-07-22 | E Ink Corporation | Spatially variable dielectric layers for digital microfluidics |
WO2021154627A1 (en) * | 2020-01-27 | 2021-08-05 | E Ink Corporation | Method for degassing liquid droplets by electrowetting actuation at higher temperatures |
JP2023513832A (en) | 2020-02-18 | 2023-04-03 | ヌークレラ ヌクリークス, リミテッド | Adaptive gate drive for high frequency AC drive of EWOD arrays |
JP2023514278A (en) | 2020-02-19 | 2023-04-05 | ヌークレラ ヌクリークス, リミテッド | Latched Transistor Drive for High Frequency AC Drive of EWoD Arrays |
US11596946B2 (en) | 2020-04-27 | 2023-03-07 | Nuclera Nucleics Ltd. | Segmented top plate for variable driving and short protection for digital microfluidics |
EP4153775A1 (en) | 2020-05-22 | 2023-03-29 | 10X Genomics, Inc. | Simultaneous spatio-temporal measurement of gene expression and cellular activity |
US11200823B1 (en) * | 2020-06-11 | 2021-12-14 | Shenzhen China Star Optoelectronics Semiconductor Display Technology Co., Ltd. | Microarray adsorption substrate, driving circuit, and display device |
CN111725125B (en) * | 2020-06-11 | 2023-07-04 | 深圳市华星光电半导体显示技术有限公司 | Microarray adsorption substrate, driving circuit and display device |
US20230304066A1 (en) | 2020-09-04 | 2023-09-28 | Baebies, Inc. | Microfluidic based assay for unbound bilirubin |
WO2022074399A1 (en) | 2020-10-08 | 2022-04-14 | Nuclera Nucleics Ltd | Electrowetting system and method for reagent-specific driving ewod arrays in microfluidic systems |
TW202235160A (en) | 2020-11-04 | 2022-09-16 | 英商核酸有限公司 | Dielectric layers for digital microfluidic devices |
Citations (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US20010026935A1 (en) | 1993-11-01 | 2001-10-04 | Nanogen, Inc. | Circuits for the control of output current in an electronic device for performing active biological operations |
Family Cites Families (26)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
FR2561404B1 (en) * | 1984-03-16 | 1988-04-08 | Thomson Csf | ELECTRICALLY CONTROLLED DEVICE FOR MOVING A FLUID |
US4583824A (en) | 1984-10-10 | 1986-04-22 | University Of Rochester | Electrocapillary devices |
US5770029A (en) | 1996-07-30 | 1998-06-23 | Soane Biosciences | Integrated electrophoretic microdevices |
US5858188A (en) | 1990-02-28 | 1999-01-12 | Aclara Biosciences, Inc. | Acrylic microchannels and their use in electrophoretic applications |
US5750015A (en) | 1990-02-28 | 1998-05-12 | Soane Biosciences | Method and device for moving molecules by the application of a plurality of electrical fields |
GB2245708A (en) | 1990-06-29 | 1992-01-08 | Philips Electronic Associated | Touch sensor array systems |
EP0574213B1 (en) | 1992-06-08 | 1999-03-24 | Synaptics, Inc. | Object position detector |
US5965452A (en) * | 1996-07-09 | 1999-10-12 | Nanogen, Inc. | Multiplexed active biologic array |
US5632876A (en) | 1995-06-06 | 1997-05-27 | David Sarnoff Research Center, Inc. | Apparatus and methods for controlling fluid flow in microchannels |
AU711564B2 (en) * | 1996-04-17 | 1999-10-14 | Motorola, Inc. | Transistor-based molecular detection apparatus and method |
US6136212A (en) | 1996-08-12 | 2000-10-24 | The Regents Of The University Of Michigan | Polymer-based micromachining for microfluidic devices |
JP3831492B2 (en) * | 1997-09-19 | 2006-10-11 | ゼロックス コーポレイション | Display device |
FR2769375B1 (en) | 1997-10-08 | 2001-01-19 | Univ Joseph Fourier | VARIABLE FOCAL LENS |
US6318970B1 (en) | 1998-03-12 | 2001-11-20 | Micralyne Inc. | Fluidic devices |
US6565727B1 (en) * | 1999-01-25 | 2003-05-20 | Nanolytics, Inc. | Actuators for microfluidics without moving parts |
US6294063B1 (en) * | 1999-02-12 | 2001-09-25 | Board Of Regents, The University Of Texas System | Method and apparatus for programmable fluidic processing |
IT1309430B1 (en) | 1999-05-18 | 2002-01-23 | Guerrieri Roberto | METHOD AND APPARATUS FOR HANDLING PARTICLES BY MEANS OF ELECTROPHORESIS |
US6449081B1 (en) | 1999-06-16 | 2002-09-10 | Canon Kabushiki Kaisha | Optical element and optical device having it |
EP2202001A3 (en) | 2000-02-23 | 2011-05-18 | Zyomyx, Inc. | Microfluidic devices and methods |
US6733645B1 (en) * | 2000-04-18 | 2004-05-11 | Caliper Technologies Corp. | Total analyte quantitation |
AU2001280796A1 (en) | 2000-07-25 | 2002-02-05 | The Regents Of The University Of California | Electrowetting-driven micropumping |
US6773566B2 (en) | 2000-08-31 | 2004-08-10 | Nanolytics, Inc. | Electrostatic actuators for microfluidics and methods for using same |
JP4184078B2 (en) | 2000-11-09 | 2008-11-19 | コーニンクレッカ フィリップス エレクトロニクス エヌ ヴィ | Multiple fluid elements with fluid levels controllable by means of matrix addressing |
US6989234B2 (en) | 2002-09-24 | 2006-01-24 | Duke University | Method and apparatus for non-contact electrostatic actuation of droplets |
US7329545B2 (en) | 2002-09-24 | 2008-02-12 | Duke University | Methods for sampling a liquid flow |
US6911132B2 (en) | 2002-09-24 | 2005-06-28 | Duke University | Apparatus for manipulating droplets by electrowetting-based techniques |
-
2002
- 2002-11-26 EP EP02794050A patent/EP1450956A2/en not_active Withdrawn
- 2002-11-26 WO PCT/US2002/038047 patent/WO2003045556A2/en active Application Filing
- 2002-11-26 US US10/305,429 patent/US7163612B2/en not_active Expired - Fee Related
- 2002-11-26 CA CA2472029A patent/CA2472029C/en not_active Expired - Fee Related
- 2002-11-26 JP JP2003547050A patent/JP2005510347A/en active Pending
- 2002-11-26 AU AU2002359508A patent/AU2002359508A1/en not_active Abandoned
Patent Citations (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US20010026935A1 (en) | 1993-11-01 | 2001-10-04 | Nanogen, Inc. | Circuits for the control of output current in an electronic device for performing active biological operations |
Cited By (129)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US7842235B2 (en) | 2004-02-14 | 2010-11-30 | Roche Diagnostics Operations, Inc. | Test element, system, and method of controlling the wetting of same |
JP2005233954A (en) * | 2004-02-16 | 2005-09-02 | Commiss Energ Atom | Device for controlling migration of droplets between two or more solid base boards |
FR2866493A1 (en) * | 2004-02-16 | 2005-08-19 | Commissariat Energie Atomique | DEVICE FOR CONTROLLING THE DISPLACEMENT OF A DROP BETWEEN TWO OR MORE SOLID SUBSTRATES |
US7531072B2 (en) | 2004-02-16 | 2009-05-12 | Commissariat A L'energie Atomique | Device for controlling the displacement of a drop between two or several solid substrates |
EP1564879A3 (en) * | 2004-02-16 | 2009-09-02 | Commissariat A L'energie Atomique | Drop traveling control device between two or more solid substrates |
JP4763690B2 (en) * | 2004-06-04 | 2011-08-31 | ユニベールシテ・デ・スジャンス・エ・テクノロジー・ドゥ・リル | Apparatus for handling droplets for biochemical analysis, method for manufacturing said apparatus and microfluidic analysis |
FR2871076A1 (en) * | 2004-06-04 | 2005-12-09 | Univ Lille Sciences Tech | DEVICE FOR LASER RADIATION DESORPTION INCORPORATING HANDLING OF THE LIQUID SAMPLE IN THE FORM OF INDIVIDUAL DROPS ENABLING THEIR CHEMICAL AND BIOCHEMICAL TREATMENT |
FR2871150A1 (en) * | 2004-06-04 | 2005-12-09 | Univ Lille Sciences Tech | DROP HANDLING DEVICE FOR BIOCHEMICAL ANALYSIS, DEVICE MANUFACTURING METHOD, AND MICROFLUIDIC ANALYSIS SYSTEM |
JP2008502882A (en) * | 2004-06-04 | 2008-01-31 | ユニベールシテ・デ・スジャンス・エ・テクノロジー・ドゥ・リル | Apparatus for handling droplets for biochemical analysis, method for manufacturing said apparatus and microfluidic analysis |
WO2006003293A3 (en) * | 2004-06-04 | 2006-09-21 | Univ Lille Sciences Tech | Device for handling drops for biochemical analysis, method for producing said device and a system for microfludic analysis |
KR101179411B1 (en) | 2004-06-04 | 2012-09-07 | 상뜨르 나쇼날 드 라 러쉐르쉬 샹띠피끄 | Device for handling drops for biochemical analysis, method for producing said device and a system for microfluidic analysis |
WO2006003292A1 (en) * | 2004-06-04 | 2006-01-12 | Universite Des Sciences Et Technologies De Lille | Laser radiation desorption device for manipulating a liquid sample in the form of individual drops, thereby making it possible to carry out the chemical and biological treatment thereof |
US7750290B2 (en) | 2004-06-04 | 2010-07-06 | Universite Des Sciences Et Technologies De Lille | Laser radiation desorption device for manipulating a liquid sample in the form of individual drops, thereby making it possible to carry out the chemical and biological treatment thereof |
WO2006003293A2 (en) * | 2004-06-04 | 2006-01-12 | Universite Des Sciences Et Technologies De Lille | Device for handling drops for biochemical analysis, method for producing said device and a system for microfludic analysis |
FR2867268A1 (en) * | 2004-07-01 | 2005-09-09 | Commissariat Energie Atomique | Determining at least one geometric property of a drop of liquid by observing it on a design of known dimensions, for control of chemical and biological microfluids |
US8685217B2 (en) | 2004-07-07 | 2014-04-01 | Silicon Biosystems S.P.A. | Method and apparatus for the separation and quantification of particles |
US8388823B2 (en) | 2004-07-07 | 2013-03-05 | Silicon Biosystems S.P.A. | Method and apparatus for the separation and quantification of particles |
EP1627685A1 (en) * | 2004-08-17 | 2006-02-22 | Hitachi High-Technologies Corporation | Microfuidic-chip for chemical analysis based on electrowetting |
WO2006054198A1 (en) * | 2004-11-20 | 2006-05-26 | Koninklijke Philips Electronics N.V. | Controllable optical component |
JP2006300946A (en) * | 2005-04-19 | 2006-11-02 | Commissariat A L'energie Atomique | System for transferring method and mass of micro fluid between two types of immiscible phases |
US8236156B2 (en) | 2005-04-19 | 2012-08-07 | Commissariat A L'energie Atomique | Microfluidic method and device for transferring mass between two immiscible phases |
US7919330B2 (en) | 2005-06-16 | 2011-04-05 | Advanced Liquid Logic, Inc. | Method of improving sensor detection of target molcules in a sample within a fluidic system |
WO2006138543A1 (en) * | 2005-06-16 | 2006-12-28 | Core-Microsolutions, Inc. | Biosensor detection by means of droplet driving, agitation, and evaporation |
WO2007010367A2 (en) * | 2005-07-19 | 2007-01-25 | Silicon Biosystems S.P.A. | Method and apparatus for the manipulation and/or the detection of particles |
US8641880B2 (en) | 2005-07-19 | 2014-02-04 | Silicon Biosystems S.P.A. | Method and apparatus for the manipulation and/or the detection of particles |
US9719960B2 (en) | 2005-07-19 | 2017-08-01 | Menarini Silicon Biosystems S.P.A. | Method and apparatus for the manipulation and/or the detection of particles |
CN102225367A (en) * | 2005-07-19 | 2011-10-26 | 硅生物系统股份公司 | Method and apparatus for the manipulation and/or the detection of particles |
WO2007010367A3 (en) * | 2005-07-19 | 2007-04-26 | Silicon Biosystems Spa | Method and apparatus for the manipulation and/or the detection of particles |
EP3851196A1 (en) * | 2005-07-19 | 2021-07-21 | Menarini Silicon Biosystems S.p.A. | Method and apparatus for the manipulation and/or the detection of particles |
WO2007022311A3 (en) * | 2005-08-17 | 2007-08-02 | Univ Cincinnati | Electrofluidic textiles and cleaning implements using such electrofluidic textiles |
WO2007022311A2 (en) * | 2005-08-17 | 2007-02-22 | The University Of Cincinnati | Electrofluidic textiles and cleaning implements using such electrofluidic textiles |
US8308927B2 (en) | 2005-08-17 | 2012-11-13 | University Of Cincinnati | Electrofluidic textiles and cleaning implements using such electrofluidic textiles |
US9211490B2 (en) | 2005-08-17 | 2015-12-15 | University Of Cincinnati | Electrofluidic textiles and cleaning implements using such electrofluidic textiles |
US8679315B2 (en) | 2005-10-26 | 2014-03-25 | Silicon Biosystems S.P.A. | Method and apparatus for characterizing and counting particles, in particular, biological particles |
US8992754B2 (en) | 2005-10-26 | 2015-03-31 | Silicon Biosystems S.P.A. | Method and apparatus for the characterizing and counting particles, in particular, biological particles |
US10092904B2 (en) | 2006-03-27 | 2018-10-09 | Menarini Silicon Biosystems S.P.A. | Method and apparatus for the processing and/or analysis and/or selection of particles, in particular biological particles |
US8679856B2 (en) | 2006-03-27 | 2014-03-25 | Silicon Biosystems S.P.A. | Method and apparatus for the processing and/or analysis and/or selection of particles, in particular biological particles |
US9581528B2 (en) | 2006-03-27 | 2017-02-28 | Menarini Silicon Biosystems S.P.A. | Method and apparatus for the processing and/or analysis and/or selection of particles, in particular, biological particles |
US9358551B2 (en) | 2006-04-13 | 2016-06-07 | Advanced Liquid Logic, Inc. | Bead manipulation techniques |
US9476856B2 (en) | 2006-04-13 | 2016-10-25 | Advanced Liquid Logic, Inc. | Droplet-based affinity assays |
US9050606B2 (en) | 2006-04-13 | 2015-06-09 | Advanced Liquid Logic, Inc. | Bead manipulation techniques |
US9205433B2 (en) | 2006-04-13 | 2015-12-08 | Advanced Liquid Logic, Inc. | Bead manipulation techniques |
US8637317B2 (en) | 2006-04-18 | 2014-01-28 | Advanced Liquid Logic, Inc. | Method of washing beads |
US10809254B2 (en) | 2006-04-18 | 2020-10-20 | Advanced Liquid Logic, Inc. | Manipulation of beads in droplets and methods for manipulating droplets |
US8389297B2 (en) | 2006-04-18 | 2013-03-05 | Duke University | Droplet-based affinity assay device and system |
US10078078B2 (en) | 2006-04-18 | 2018-09-18 | Advanced Liquid Logic, Inc. | Bead incubation and washing on a droplet actuator |
US8492168B2 (en) | 2006-04-18 | 2013-07-23 | Advanced Liquid Logic Inc. | Droplet-based affinity assays |
US9086345B2 (en) | 2006-04-18 | 2015-07-21 | Advanced Liquid Logic, Inc. | Manipulation of beads in droplets and methods for manipulating droplets |
US9395361B2 (en) | 2006-04-18 | 2016-07-19 | Advanced Liquid Logic, Inc. | Bead incubation and washing on a droplet actuator |
US9395329B2 (en) | 2006-04-18 | 2016-07-19 | Advanced Liquid Logic, Inc. | Droplet-based particle sorting |
US9097662B2 (en) | 2006-04-18 | 2015-08-04 | Advanced Liquid Logic, Inc. | Droplet-based particle sorting |
US8637324B2 (en) | 2006-04-18 | 2014-01-28 | Advanced Liquid Logic, Inc. | Bead incubation and washing on a droplet actuator |
US10139403B2 (en) | 2006-04-18 | 2018-11-27 | Advanced Liquid Logic, Inc. | Manipulation of beads in droplets and methods for manipulating droplets |
US9377455B2 (en) | 2006-04-18 | 2016-06-28 | Advanced Liquid Logic, Inc | Manipulation of beads in droplets and methods for manipulating droplets |
US10585090B2 (en) | 2006-04-18 | 2020-03-10 | Advanced Liquid Logic, Inc. | Bead incubation and washing on a droplet actuator |
US9494498B2 (en) | 2006-04-18 | 2016-11-15 | Advanced Liquid Logic, Inc. | Manipulation of beads in droplets and methods for manipulating droplets |
US11255809B2 (en) | 2006-04-18 | 2022-02-22 | Advanced Liquid Logic, Inc. | Droplet-based surface modification and washing |
US11525827B2 (en) | 2006-04-18 | 2022-12-13 | Advanced Liquid Logic, Inc. | Bead incubation and washing on a droplet actuator |
US9243282B2 (en) | 2006-04-18 | 2016-01-26 | Advanced Liquid Logic, Inc | Droplet-based pyrosequencing |
US9081007B2 (en) | 2006-04-18 | 2015-07-14 | Advanced Liquid Logic, Inc. | Bead incubation and washing on a droplet actuator |
US8809068B2 (en) | 2006-04-18 | 2014-08-19 | Advanced Liquid Logic, Inc. | Manipulation of beads in droplets and methods for manipulating droplets |
US8845872B2 (en) | 2006-04-18 | 2014-09-30 | Advanced Liquid Logic, Inc. | Sample processing droplet actuator, system and method |
US8846410B2 (en) | 2006-04-18 | 2014-09-30 | Advanced Liquid Logic, Inc. | Bead incubation and washing on a droplet actuator |
US11789015B2 (en) | 2006-04-18 | 2023-10-17 | Advanced Liquid Logic, Inc. | Manipulation of beads in droplets and methods for manipulating droplets |
WO2008051310A2 (en) | 2006-05-09 | 2008-05-02 | Advanced Liquid Logic, Inc. | Droplet manipulation systems |
EP2021103A2 (en) * | 2006-05-09 | 2009-02-11 | Advanced Liquid Logic, Inc. | Droplet manipulation systems |
EP2021103A4 (en) * | 2006-05-09 | 2011-02-23 | Advanced Liquid Logic Inc | Droplet manipulation systems |
WO2008075053A3 (en) * | 2006-12-19 | 2008-10-23 | Cambridge Entpr Ltd | Particle transport and characterisation |
WO2008075053A2 (en) * | 2006-12-19 | 2008-06-26 | Cambridge Enterprise Limited | Particle transport and characterisation |
US9046514B2 (en) | 2007-02-09 | 2015-06-02 | Advanced Liquid Logic, Inc. | Droplet actuator devices and methods employing magnetic beads |
US10379112B2 (en) | 2007-02-09 | 2019-08-13 | Advanced Liquid Logic, Inc. | Droplet actuator devices and methods employing magnetic beads |
WO2008117210A1 (en) * | 2007-03-23 | 2008-10-02 | Koninklijke Philips Electronics N.V. | Integrated microfluidic device with local temperature control |
WO2009019658A2 (en) * | 2007-08-09 | 2009-02-12 | Koninklijke Philips Electronics N.V. | Integrated microfluidic device with local temperature control |
WO2009019658A3 (en) * | 2007-08-09 | 2009-04-02 | Koninkl Philips Electronics Nv | Integrated microfluidic device with local temperature control |
WO2009053880A1 (en) * | 2007-10-22 | 2009-04-30 | Koninklijke Philips Electronics N.V. | Device and method for the monitoring of the movement of cells |
EP2053396A1 (en) * | 2007-10-22 | 2009-04-29 | Koninklijke Philips Electronics N.V. | Device and method for the monitoring of the movement of cells |
US9310287B2 (en) | 2007-10-29 | 2016-04-12 | Silicon Biosystems S.P.A. | Method and apparatus for the identification and handling of particles |
US10648897B2 (en) | 2007-10-29 | 2020-05-12 | Menarini Silicon Biosystems S.P.A. | Method and apparatus for the identification and handling of particles |
EP2250249A4 (en) * | 2008-02-11 | 2013-12-04 | Univ Toronto | Cell culture and cell assays using digital microfluidics |
EP2250249A1 (en) * | 2008-02-11 | 2010-11-17 | Aaron R. Wheeler | Cell culture and cell assays using digital microfluidics |
US9249443B2 (en) | 2008-02-11 | 2016-02-02 | The Governing Council Of The University Of Toronto | Cell culture and cell assays using digital microfluidics |
US8728291B2 (en) | 2008-02-11 | 2014-05-20 | The Governing Council Of The University Of Toronto | Droplet-based cell culture and cell assays using digital microfluidics |
WO2009101584A2 (en) * | 2008-02-15 | 2009-08-20 | Koninklijke Philips Electronics N.V. | Driver system for lab-on-a-chip cartridges |
WO2009101584A3 (en) * | 2008-02-15 | 2009-12-03 | Koninklijke Philips Electronics N.V. | Driver system for lab-on-a-chip cartridges |
US9039973B2 (en) | 2008-10-10 | 2015-05-26 | The Governing Council Of The University Of Toronto | Hybrid digital and channel microfluidic devices and methods of use thereof |
US10234447B2 (en) | 2008-11-04 | 2019-03-19 | Menarini Silicon Biosystems S.P.A. | Method for identification, selection and analysis of tumour cells |
US10895575B2 (en) | 2008-11-04 | 2021-01-19 | Menarini Silicon Biosystems S.P.A. | Method for identification, selection and analysis of tumour cells |
US9192943B2 (en) | 2009-03-17 | 2015-11-24 | Silicon Biosystems S.P.A. | Microfluidic device for isolation of cells |
US11000850B2 (en) | 2010-05-05 | 2021-05-11 | The Governing Council Of The University Of Toronto | Method of processing dried samples using digital microfluidic device |
US10232374B2 (en) | 2010-05-05 | 2019-03-19 | Miroculus Inc. | Method of processing dried samples using digital microfluidic device |
US8654571B2 (en) | 2010-07-06 | 2014-02-18 | Sharp Kabushiki Kaisha | Static random-access cell, active matrix device and array element circuit |
EP2404675A1 (en) * | 2010-07-06 | 2012-01-11 | Sharp Kabushiki Kaisha | Array element circuit and active matrix device |
US20120007608A1 (en) * | 2010-07-06 | 2012-01-12 | Sharp Kabushiki Kaisha | Array element circuit and active matrix device |
CN102389840A (en) * | 2010-07-06 | 2012-03-28 | 夏普株式会社 | Array element circuit and active matrix device |
US8547111B2 (en) | 2010-07-06 | 2013-10-01 | Sharp Kabushiki Kaisha | Array element circuit and active matrix device |
US8653832B2 (en) | 2010-07-06 | 2014-02-18 | Sharp Kabushiki Kaisha | Array element circuit and active matrix device |
WO2012084707A1 (en) * | 2010-12-20 | 2012-06-28 | Technische Universität Ilmenau | Micropump for generating a fluid flow, pump system, and microchannel system |
US9950322B2 (en) | 2010-12-22 | 2018-04-24 | Menarini Silicon Biosystems S.P.A. | Microfluidic device for the manipulation of particles |
US11921028B2 (en) | 2011-10-28 | 2024-03-05 | Menarini Silicon Biosystems S.P.A. | Method and device for optical analysis of particles at low temperatures |
US10376878B2 (en) | 2011-12-28 | 2019-08-13 | Menarini Silicon Biosystems S.P.A. | Devices, apparatus, kit and method for treating a biological sample |
WO2013137499A1 (en) * | 2012-03-14 | 2013-09-19 | 엘지전자 주식회사 | Biosensor |
EP2867645A4 (en) * | 2012-06-27 | 2016-06-29 | Advanced Liquid Logic Inc | Techniques and droplet actuator designs for reducing bubble formation |
US9815061B2 (en) | 2012-06-27 | 2017-11-14 | Advanced Liquid Logic, Inc. | Techniques and droplet actuator designs for reducing bubble formation |
US9169573B2 (en) | 2013-01-23 | 2015-10-27 | Sharp Kabushiki Kaisha | AM-EWOD device and method of driving with variable voltage AC driving |
EP2759342A3 (en) * | 2013-01-23 | 2014-08-13 | Sharp Kabushiki Kaisha | AM-EWOD device and method of driving with AC voltage |
US9945738B2 (en) | 2013-03-15 | 2018-04-17 | Genmark Diagnostics, Inc. | Devices and methods for monitoring and controlling temperature in a microfluidic environment |
US11156508B2 (en) | 2013-03-15 | 2021-10-26 | Roche Molecular Systems, Inc. | Devices and methods for monitoring and controlling temperature in a microfluidic environment |
US10464067B2 (en) | 2015-06-05 | 2019-11-05 | Miroculus Inc. | Air-matrix digital microfluidics apparatuses and methods for limiting evaporation and surface fouling |
US11471888B2 (en) | 2015-06-05 | 2022-10-18 | Miroculus Inc. | Evaporation management in digital microfluidic devices |
US10695762B2 (en) | 2015-06-05 | 2020-06-30 | Miroculus Inc. | Evaporation management in digital microfluidic devices |
US11097276B2 (en) | 2015-06-05 | 2021-08-24 | mirOculus, Inc. | Air-matrix digital microfluidics apparatuses and methods for limiting evaporation and surface fouling |
US11890617B2 (en) | 2015-06-05 | 2024-02-06 | Miroculus Inc. | Evaporation management in digital microfluidic devices |
US11298700B2 (en) | 2016-08-22 | 2022-04-12 | Miroculus Inc. | Feedback system for parallel droplet control in a digital microfluidic device |
US10596572B2 (en) | 2016-08-22 | 2020-03-24 | Miroculus Inc. | Feedback system for parallel droplet control in a digital microfluidic device |
US11833516B2 (en) | 2016-12-28 | 2023-12-05 | Miroculus Inc. | Digital microfluidic devices and methods |
US11253860B2 (en) | 2016-12-28 | 2022-02-22 | Miroculus Inc. | Digital microfluidic devices and methods |
US11623219B2 (en) | 2017-04-04 | 2023-04-11 | Miroculus Inc. | Digital microfluidics apparatuses and methods for manipulating and processing encapsulated droplets |
US10408788B2 (en) | 2017-07-12 | 2019-09-10 | Sharp Life Science (Eu) Limited | Spacer for side loaded EWOD device |
US11413617B2 (en) | 2017-07-24 | 2022-08-16 | Miroculus Inc. | Digital microfluidics systems and methods with integrated plasma collection device |
US11857969B2 (en) | 2017-07-24 | 2024-01-02 | Miroculus Inc. | Digital microfluidics systems and methods with integrated plasma collection device |
US11311882B2 (en) | 2017-09-01 | 2022-04-26 | Miroculus Inc. | Digital microfluidics devices and methods of using them |
US11577244B2 (en) | 2018-09-12 | 2023-02-14 | Sharp Life Science (Eu) Limited | Microfluidic device and a method of loading fluid therein |
EP3623049A1 (en) * | 2018-09-12 | 2020-03-18 | Sharp Life Science (EU) Limited | Microfluidic device and a method of loading fluid therein |
US11517902B2 (en) | 2018-09-12 | 2022-12-06 | Sharp Life Science (Eu) Limited | Microfluidic device and a method of loading fluid therein |
US11738345B2 (en) | 2019-04-08 | 2023-08-29 | Miroculus Inc. | Multi-cartridge digital microfluidics apparatuses and methods of use |
US11524298B2 (en) | 2019-07-25 | 2022-12-13 | Miroculus Inc. | Digital microfluidics devices and methods of use thereof |
FR3127810A1 (en) | 2021-10-01 | 2023-04-07 | Commissariat à l'Energie Atomique et aux Energies Alternatives | Drop sorting process |
US11772093B2 (en) | 2022-01-12 | 2023-10-03 | Miroculus Inc. | Methods of mechanical microfluidic manipulation |
US11857961B2 (en) | 2022-01-12 | 2024-01-02 | Miroculus Inc. | Sequencing by synthesis using mechanical compression |
Also Published As
Publication number | Publication date |
---|---|
CA2472029C (en) | 2014-04-15 |
WO2003045556A3 (en) | 2004-04-15 |
CA2472029A1 (en) | 2003-06-05 |
JP2005510347A (en) | 2005-04-21 |
EP1450956A2 (en) | 2004-09-01 |
US20030164295A1 (en) | 2003-09-04 |
US7163612B2 (en) | 2007-01-16 |
AU2002359508A8 (en) | 2003-06-10 |
AU2002359508A1 (en) | 2003-06-10 |
Similar Documents
Publication | Publication Date | Title |
---|---|---|
CA2472029C (en) | Method, apparatus and article for microfluidic control via electrowetting, for chemical, biochemical and biological assays and the like | |
US20040231987A1 (en) | Method, apparatus and article for microfluidic control via electrowetting, for chemical, biochemical and biological assays and the like | |
EP2148838B1 (en) | Electrowetting based digital microfluidics | |
Kong et al. | Motorized actuation system to perform droplet operations on printed plastic sheets | |
EP1998886B1 (en) | Microelectronic device with heating array | |
US20110220505A1 (en) | Droplet manipulations on ewod microelectrode array architecture | |
EP2884272B1 (en) | Method for splitting droplets by electrowetting-based techniques | |
WO2013066441A2 (en) | Digital microfluidic platform for actuating and heating individual liquid droplets | |
US10695763B2 (en) | Fluid extraction from a microfluidic device | |
Umapathi et al. | Scaling electrowetting with printed circuit boards for large area droplet manipulation | |
WO2021168131A1 (en) | Adaptive gate driving for high frequency ac driving of ewod arrays | |
Shaik et al. | Thin-film-transistor array: An exploratory attempt for high throughput cell manipulation using electrowetting principle | |
Madison et al. | Fluid transport in partially shielded electrowetting on dielectric digital microfluidic devices | |
WO2021113485A1 (en) | Variable electrode size area arrays on thin-film transistor based digital microfluidic devices for fine droplet manipulation | |
Schultz et al. | Investigation of Laplace barriers for arrayed electrowetting lab-on-a-chip | |
WO2021003631A1 (en) | Microfluidic droplet printing system-based digital pcr detection method and application thereof | |
Luo et al. | Programmable high integration and resolution digital microfluidic device driven by thin film transistor arrays | |
CN114632561A (en) | Hybrid digital microfluidic chip and droplet driving method | |
CN116367921A (en) | Digital microfluidic chip, driving method thereof and digital microfluidic device | |
Cheng et al. | Simultaneous multiple-droplet generation with meniscus filling on digital microfluidics chip | |
Ho et al. | Driving and controller design of digital microdroplet | |
US20130210070A1 (en) | Microfluidic device and method for controlling interaction between liquids | |
Ugsornrat et al. | Experimental study of digital microfluidic biochip | |
WO2023247948A1 (en) | Controlled reservoir filling | |
WO2023285821A1 (en) | A method of forming arrays of droplets |
Legal Events
Date | Code | Title | Description |
---|---|---|---|
AK | Designated states |
Kind code of ref document: A2 Designated state(s): AE AG AL AM AT AU AZ BA BB BG BR BY BZ CA CH CN CO CR CU CZ DE DK DM DZ EC EE ES FI GB GD GE GH GM HR HU ID IL IN IS JP KE KG KP KR KZ LC LK LR LS LT LU LV MA MD MG MK MN MW MX MZ NO NZ OM PH PL PT RO RU SC SD SE SG SI SK SL TJ TM TN TR TT TZ UA UG US UZ VC VN YU ZA ZM ZW |
|
AL | Designated countries for regional patents |
Kind code of ref document: A2 Designated state(s): GH GM KE LS MW MZ SD SL SZ TZ UG ZM ZW AM AZ BY KG KZ MD RU TJ TM AT BE BG CH CY CZ DE DK EE ES FI FR GB GR IE IT LU MC NL PT SE SK TR BF BJ CF CG CI CM GA GN GQ GW ML MR NE SN TD TG |
|
121 | Ep: the epo has been informed by wipo that ep was designated in this application | ||
WWE | Wipo information: entry into national phase |
Ref document number: 2003547050 Country of ref document: JP |
|
REEP | Request for entry into the european phase |
Ref document number: 2002794050 Country of ref document: EP |
|
WWE | Wipo information: entry into national phase |
Ref document number: 2002794050 Country of ref document: EP |
|
WWE | Wipo information: entry into national phase |
Ref document number: 2472029 Country of ref document: CA |
|
WWP | Wipo information: published in national office |
Ref document number: 2002794050 Country of ref document: EP |