WO2003012098A1 - Levure modifiee presentant un potentiel secretoire eleve de proteine etrangere et procede permettant de produire une proteine etrangere a l'aide de la levure - Google Patents

Levure modifiee presentant un potentiel secretoire eleve de proteine etrangere et procede permettant de produire une proteine etrangere a l'aide de la levure Download PDF

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Publication number
WO2003012098A1
WO2003012098A1 PCT/JP2002/007857 JP0207857W WO03012098A1 WO 2003012098 A1 WO2003012098 A1 WO 2003012098A1 JP 0207857 W JP0207857 W JP 0207857W WO 03012098 A1 WO03012098 A1 WO 03012098A1
Authority
WO
WIPO (PCT)
Prior art keywords
yeast
foreign protein
producing
showing high
high secretion
Prior art date
Application number
PCT/JP2002/007857
Other languages
English (en)
French (fr)
Inventor
Toshihiro Komeda
Keiji Kondo
Original Assignee
Kirin Beer Kabushiki Kaisha
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Kirin Beer Kabushiki Kaisha filed Critical Kirin Beer Kabushiki Kaisha
Publication of WO2003012098A1 publication Critical patent/WO2003012098A1/ja

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
    • C12N15/81—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts
    • C12N15/815—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts for yeasts other than Saccharomyces
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/14—Fungi; Culture media therefor
    • C12N1/16—Yeasts; Culture media therefor
    • C12N1/165—Yeast isolates
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
    • C12P21/02—Preparation of peptides or proteins having a known sequence of two or more amino acids, e.g. glutathione
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
    • C12R2001/00—Microorganisms ; Processes using microorganisms
    • C12R2001/645—Fungi ; Processes using fungi
    • C12R2001/72—Candida
PCT/JP2002/007857 2001-08-01 2002-08-01 Levure modifiee presentant un potentiel secretoire eleve de proteine etrangere et procede permettant de produire une proteine etrangere a l'aide de la levure WO2003012098A1 (fr)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
JP2001233342A JP2003047471A (ja) 2001-08-01 2001-08-01 異種タンパク質の分泌生産に優れた改変酵母およびその酵母を用いた異種タンパク質の製造法
JP2001-233342 2001-08-01

Publications (1)

Publication Number Publication Date
WO2003012098A1 true WO2003012098A1 (fr) 2003-02-13

Family

ID=19065125

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/JP2002/007857 WO2003012098A1 (fr) 2001-08-01 2002-08-01 Levure modifiee presentant un potentiel secretoire eleve de proteine etrangere et procede permettant de produire une proteine etrangere a l'aide de la levure

Country Status (2)

Country Link
JP (1) JP2003047471A (ja)
WO (1) WO2003012098A1 (ja)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2011078351A1 (ja) * 2009-12-25 2011-06-30 キリンホールディングス株式会社 分泌シグナル配列およびこれを利用したタンパク質発現系

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2009240185A (ja) * 2008-03-28 2009-10-22 Toyota Central R&D Labs Inc 外来性タンパク質の高生産形質転換体及びその利用

Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1995023857A1 (en) * 1994-03-05 1995-09-08 Delta Biotechnology Limited Yeast strains and modified albumins
WO1998007873A1 (fr) * 1996-08-23 1998-02-26 Kirin Beer Kabushiki Kaisha Vecteurs de levure et procede de production de proteines les utilisant
WO1998014600A1 (es) * 1996-10-03 1998-04-09 Centro De Ingenieria Genetica Y Biotecnologia (Cigb) SISTEMA DE TRANSFORMACION EN $i(CANDIDA UTILIS)
WO2000014259A1 (fr) * 1998-09-04 2000-03-16 Kirin Beer Kabushiki Kaisha Souches de candida boidinii presentant une activite protease reduite et utilisation de ces dernieres en tant qu'hote pour produire une proteine exogene

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1995023857A1 (en) * 1994-03-05 1995-09-08 Delta Biotechnology Limited Yeast strains and modified albumins
WO1998007873A1 (fr) * 1996-08-23 1998-02-26 Kirin Beer Kabushiki Kaisha Vecteurs de levure et procede de production de proteines les utilisant
WO1998014600A1 (es) * 1996-10-03 1998-04-09 Centro De Ingenieria Genetica Y Biotecnologia (Cigb) SISTEMA DE TRANSFORMACION EN $i(CANDIDA UTILIS)
WO2000014259A1 (fr) * 1998-09-04 2000-03-16 Kirin Beer Kabushiki Kaisha Souches de candida boidinii presentant une activite protease reduite et utilisation de ces dernieres en tant qu'hote pour produire une proteine exogene

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
ROTHSTEIN RODNEY: "Targeting, disruption, replacement and allele rescue: integrative DNA transformation in yeast", METHODS IN ENZYMOLOGY, vol. 1974, 1991, pages 281 - 301, XP002958952 *

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2011078351A1 (ja) * 2009-12-25 2011-06-30 キリンホールディングス株式会社 分泌シグナル配列およびこれを利用したタンパク質発現系

Also Published As

Publication number Publication date
JP2003047471A (ja) 2003-02-18

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