CN105319197A - Liquid drop micro-fluidic chip based on microlens array - Google Patents
Liquid drop micro-fluidic chip based on microlens array Download PDFInfo
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- CN105319197A CN105319197A CN201510868657.4A CN201510868657A CN105319197A CN 105319197 A CN105319197 A CN 105319197A CN 201510868657 A CN201510868657 A CN 201510868657A CN 105319197 A CN105319197 A CN 105319197A
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- microlens array
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Abstract
The invention relates to a liquid drop micro-fluidic chip based on a microlens array. The liquid drop micro-fluidic chip comprises a chip body, a reflection membrane layer, a light transmission plate and a microlens, wherein at least one flow channel is formed in the surface of the chip body; the reflection membrane layer is arranged on an inner wall of the flow channel; the light transmission plate covers the flow channel; and the microlens is arranged on the light transmission plate and is located just above the flow channel. By using a light energy gathering property of the microlens array, a microlens array technology is combined with the liquid drop micro-fluidic chip, so that the intensity of fluorescent light received by a detector can be extremely enhanced; and by optimally designing a liquid drop flowing pipeline of a detection region and improving the quantity of parallel detection channels, the detection flux can be improved by 1-2 orders of magnitudes as compared with that of a traditional flow type technology.
Description
Technical field
The present invention relates to a kind of micro-fluidic chip, particularly a kind of drop micro-fluidic chip based on microlens array.
Background technology
Drop micro-fluidic chip is the technology of a kind of manipulation micro liquid newly grown up on micro-fluidic chip basis in recent years.Compared with conventional microchannel chip, the volume of microlayer model is less by (10
-9l-10
-12l), can manipulate flexibly, size uniformity, shape is variable, and heat and mass transfer performance is outstanding, and has the good potentiality of high throughput analysis.Drop microflow control technique is applied in biochemical analysis field, the normal use interested cell of fluorescence labeling or molecule, by identifying testing concentration in band fluorescence drop detection sample.Along with the development of drop microflow control technique, the generating rate of drop improves constantly, and identifies that the quick detection tool of band fluorescence drop to sample is of great significance accurately, high flux.
The universal method of current drop identification is traditional sequence detection fluorescence drop method of identification, allows drop order flow through microchannel, detects successively in surveyed area.The mode sensitivity of sequence detection is higher, but in order to prevent droplet rupture, detect flux and limit by rate of flow of fluid, theoretical upper limit is at about thousands of drops/sec.The Parallel detection of fluorescence drop is more and more subject to people's attention in recent years, and have report to utilize CCD wide field fluorescence imaging to detect a large amount of drop, the method device is easy simultaneously, detection flux is high, but because fluorescence signal is faint, need the longer sampling time, signal to noise ratio (S/N ratio) is low.
Summary of the invention
For the deficiency that prior art exists, the object of the present invention is to provide a kind of drop micro-fluidic chip based on microlens array, to solve the weak problem of fluorescence signal, thus the detection demand of large flux can be met.
For achieving the above object, the present invention is achieved through the following technical solutions:
Based on a drop micro-fluidic chip for microlens array, comprising:
Chip body, its surface is provided with at least one runner;
Reflective coating, it is arranged at the inwall of described runner;
Light-passing board, its lid is pressed in above described runner;
Lenticule, it to be arranged on described light-passing board and to be positioned at directly over described runner.
Preferably, the described drop micro-fluidic chip based on microlens array, wherein, described reflective coating is metal level.
Preferably, the described drop micro-fluidic chip based on microlens array, wherein, described light-passing board is glass or light-transmissive resin.
Preferably, the described drop micro-fluidic chip based on microlens array, wherein, described lenticule is plano-convex lens.
Preferably, the described drop micro-fluidic chip based on microlens array, wherein, in described plano-convex lens, flat one side is just to described runner.
Preferably, the described drop micro-fluidic chip based on microlens array, wherein, the thickness of described light-passing board is 150 ~ 250 μm.
Preferably, the described drop micro-fluidic chip based on microlens array, wherein, the diameter of described runner is 80 ~ 120 μm.
Preferably, the described drop micro-fluidic chip based on microlens array, wherein, described lenticular radius-of-curvature is 100 ~ 150 μm.
Preferably, the described drop micro-fluidic chip based on microlens array, wherein, described lenticular focal length is 220 ~ 280 μm.
The invention has the beneficial effects as follows: this case utilizes the light cumulative characteristic of microlens array, microlens array technology is combined with drop micro-fluidic chip, greatly can strengthen the intensity that detector receives fluorescence, improve parallel detection number of channels by the droplet flow pipeline of optimal design surveyed area, can improve compared with conventional flow technology and detect the flux 1-2 order of magnitude.
Accompanying drawing explanation
Fig. 1 is the cut-open view of the drop micro-fluidic chip based on microlens array.
Fig. 2 is the vertical view of the drop micro-fluidic chip based on microlens array.
Fig. 3 is the structural representation of the detection system of application this case drop micro-fluidic chip.
Embodiment
Below in conjunction with accompanying drawing, the present invention is described in further detail, can implement according to this with reference to instructions word to make those skilled in the art.
As Figure 1-3, the drop micro-fluidic chip based on microlens array of an embodiment is listed in this case, comprising:
Chip body 1, its surface is provided with at least one runner 2;
Reflective coating 3, it is arranged at the inwall of runner 2;
Light-passing board 4, its lid is pressed in above runner 2; For runner 2 being sealed to a closed pipeline;
Lenticule 5, it to be arranged on light-passing board 4 and to be positioned at directly over runner 2.Lenticule utilizes ultraviolet photolithographic technology to make photoresist structure in substrate, then utilizes hot reflux technical matters change photoresist structure into lenticule and make.During detection, after exciting light converges through lenticule 5, to be irradiated in runner 2 on the drop of flowing, again to send after lenticule 5 converges after the fluorescence excitation of drop is reflected by reflective coating 3.
In the above-described embodiments, reflective coating 3 is preferably metal level.The effect of metal level strengthens the fluorescence signal intensity being stimulated and producing.
In the above-described embodiments, light-passing board 4 is preferably glass or light-transmissive resin.
In the above-described embodiments, lenticule 5 is preferably plano-convex lens.
In the above-described embodiments, in plano-convex lens, flat one side is just to runner 2, and convex one side is back to runner 2.
In the above-described embodiments, the thickness of light-passing board 4 is preferably 150 ~ 250 μm, if exceed preferred scope, by the fluorescence intensity affecting ccd sensor and finally receive.
In the above-described embodiments, the diameter of runner 2 is preferably 80 ~ 120 μm, if exceed preferred scope, by the fluorescence intensity affecting ccd sensor and finally receive.
In the above-described embodiments, the radius-of-curvature of lenticule 5 is preferably 100 ~ 150 μm, if exceed preferred scope, by the fluorescence intensity affecting ccd sensor and finally receive.
In the above-described embodiments, the focal length of lenticule 5 is preferably 220 ~ 280 μm, if exceed preferred scope, by the fluorescence intensity affecting ccd sensor and finally receive.
Fig. 2 is the vertical view of micro-fluidic chip, chip body 1 is dispersed with many runners 2, after sample droplet is loaded into micro-fluidic chip from injection port 7, shunted by multistage Y type runner, enter surveyed area 6 subsequently, in surveyed area 6, arrange microlens array, fluorescence excitation is detected device and detects after lenticule 5 converges.In order to detection drop as much as possible in limited surveyed area 6, the mode of multi-channel parallel arrangement can be designed.After surveyed area 6, sample flows out micro-fluidic chip from waste liquid port 8.
Fig. 3 is the light path system schematic diagram using micro-fluidic chip to carry out drop fluoroscopic examination.Excitation source 9 be there is specific wavelength higher source luminance as laser, highlighted LED etc., excitation source 9 sends exciting light after plus lens 10 and the first optical filter 11, be irradiated on chip body 1, after focusing on through lenticule 5, excite drop to be measured to produce fluorescence.The fluorescence that drop sends enters ccd sensor 13 after the exciting light of the second optical filter 12 filtering scattering, the fluorescence signal received is converted to plane electric signal and is transferred in computer 14 by the latter, because microlens array position is fixed, the fluorescence drop of process under each micro mirror can be obtained by the pre-determined determined electrical signal intensity of pixel representing microlens array position.
Although embodiment of the present invention are open as above, but it is not restricted to listed in instructions and embodiment utilization, it can be applied to various applicable the field of the invention completely, for those skilled in the art, can easily realize other amendment, therefore do not deviating under the universal that claim and equivalency range limit, the present invention is not limited to specific details and illustrates here and the legend described.
Claims (9)
1., based on a drop micro-fluidic chip for microlens array, it is characterized in that, comprising:
Chip body, its surface is provided with at least one runner;
Reflective coating, it is arranged at the inwall of described runner;
Light-passing board, its lid is pressed in above described runner;
Lenticule, it to be arranged on described light-passing board and to be positioned at directly over described runner.
2., as claimed in claim 1 based on the drop micro-fluidic chip of microlens array, it is characterized in that, described reflective coating is metal level.
3., as claimed in claim 1 based on the drop micro-fluidic chip of microlens array, it is characterized in that, described light-passing board is glass or light-transmissive resin.
4., as claimed in claim 1 based on the drop micro-fluidic chip of microlens array, it is characterized in that, described lenticule is plano-convex lens.
5., as claimed in claim 4 based on the drop micro-fluidic chip of microlens array, it is characterized in that, in described plano-convex lens, flat one side is just to described runner.
6., as claimed in claim 1 based on the drop micro-fluidic chip of microlens array, it is characterized in that, the thickness of described light-passing board is 150 ~ 250 μm.
7., as claimed in claim 1 based on the drop micro-fluidic chip of microlens array, it is characterized in that, the diameter of described runner is 80 ~ 120 μm.
8., as claimed in claim 1 based on the drop micro-fluidic chip of microlens array, it is characterized in that, described lenticular radius-of-curvature is 100 ~ 150 μm.
9., as claimed in claim 1 based on the drop micro-fluidic chip of microlens array, it is characterized in that, described lenticular focal length is 220 ~ 280 μm.
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| CN201510868657.4A CN105319197A (en) | 2015-12-02 | 2015-12-02 | Liquid drop micro-fluidic chip based on microlens array |
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| CN201510868657.4A CN105319197A (en) | 2015-12-02 | 2015-12-02 | Liquid drop micro-fluidic chip based on microlens array |
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Cited By (9)
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| CN105954194A (en) * | 2016-04-28 | 2016-09-21 | 大连理工大学 | Light cone-based portable optical flow control type micro-imaging device and system |
| CN106546566A (en) * | 2016-10-18 | 2017-03-29 | 太原理工大学 | A kind of metal passage structure for improving fluorescent material far field irradiance efficiency |
| CN106881160A (en) * | 2017-05-03 | 2017-06-23 | 山东大学 | A kind of candida albicans bacterium micro-fluidic chip |
| CN109092378A (en) * | 2018-07-13 | 2018-12-28 | 哈尔滨工业大学 | A kind of micro-fluidic chip flow light control method based on phasmon nanostructure |
| CN109289951A (en) * | 2018-10-29 | 2019-02-01 | 深圳先进技术研究院 | Drop breakup micro-fluidic chip and application |
| CN110132931A (en) * | 2019-06-17 | 2019-08-16 | 北京百康芯生物科技有限公司 | Lamp guide passes light without lens illumination imaging device and fluidic chip detecting system |
| WO2020119562A1 (en) * | 2018-12-15 | 2020-06-18 | 中国科学院深圳先进技术研究院 | Droplet microfluidic chip for multicolor fluorescence synchronous detection |
| CN111504907A (en) * | 2020-04-09 | 2020-08-07 | 江苏师范大学 | Method for detecting luminescence signal in microchannel |
| CN112147105A (en) * | 2019-06-27 | 2020-12-29 | 深圳市帝迈生物技术有限公司 | Microfluidic sample analysis device |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN105954194A (en) * | 2016-04-28 | 2016-09-21 | 大连理工大学 | Light cone-based portable optical flow control type micro-imaging device and system |
| CN106546566A (en) * | 2016-10-18 | 2017-03-29 | 太原理工大学 | A kind of metal passage structure for improving fluorescent material far field irradiance efficiency |
| CN106546566B (en) * | 2016-10-18 | 2019-04-05 | 太原理工大学 | A kind of metal passage structure improving fluorescent material far field irradiance efficiency |
| CN106881160A (en) * | 2017-05-03 | 2017-06-23 | 山东大学 | A kind of candida albicans bacterium micro-fluidic chip |
| CN109092378A (en) * | 2018-07-13 | 2018-12-28 | 哈尔滨工业大学 | A kind of micro-fluidic chip flow light control method based on phasmon nanostructure |
| CN109289951A (en) * | 2018-10-29 | 2019-02-01 | 深圳先进技术研究院 | Drop breakup micro-fluidic chip and application |
| CN109289951B (en) * | 2018-10-29 | 2021-01-05 | 深圳先进技术研究院 | Droplet splitting microfluidic chip and application |
| WO2020119562A1 (en) * | 2018-12-15 | 2020-06-18 | 中国科学院深圳先进技术研究院 | Droplet microfluidic chip for multicolor fluorescence synchronous detection |
| CN110132931A (en) * | 2019-06-17 | 2019-08-16 | 北京百康芯生物科技有限公司 | Lamp guide passes light without lens illumination imaging device and fluidic chip detecting system |
| CN112147105A (en) * | 2019-06-27 | 2020-12-29 | 深圳市帝迈生物技术有限公司 | Microfluidic sample analysis device |
| CN111504907A (en) * | 2020-04-09 | 2020-08-07 | 江苏师范大学 | Method for detecting luminescence signal in microchannel |
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Application publication date: 20160210 |
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