CN103926421A - Autocorrelation speed detection device for cell screening - Google Patents

Autocorrelation speed detection device for cell screening Download PDF

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Publication number
CN103926421A
CN103926421A CN201410187981.5A CN201410187981A CN103926421A CN 103926421 A CN103926421 A CN 103926421A CN 201410187981 A CN201410187981 A CN 201410187981A CN 103926421 A CN103926421 A CN 103926421A
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micro
fluidic chip
grating
microlens array
spheroidal
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CN201410187981.5A
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CN103926421B (en
Inventor
周围
张旭
闫玉静
杨新颖
杨朋菲
武雪峰
王凤娇
王征
常健强
张思祥
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Hebei University of Technology
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Hebei University of Technology
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Abstract

The invention discloses an autocorrelation speed detection device for cell screening. The autocorrelation speed detection device is characterized by comprising a micro-fluidic chip, a cylindrical micro-lens array, a spheroidal micro-lens array, a micro-channel, a photosensitive device, slits, a grating, a grating fixing groove and a laser beam, wherein the cylindrical micro-lens array, the spheroidal micro-lens array, the micro-channel, the photosensitive device, the slits, the grating, the grating fixing groove and the laser beam are designed and installed inside the micro-fluidic chip. The micro-fluidic chip is a micro laboratory made of PDMS. The micro-channel is machined inside the micro-fluidic chip according to the design requirement. The spheroidal micro-lens array is inlaid on the lower layer of the plane of the micro-channel. The grating is arranged under the spheroidal micro-lens array. The two slits with the design interval and the width are formed in the middle of the grating. A fixing groove for the grating is fixed inside the micro-fluidic chip. The fixing groove for the grating and the micro-fluidic chip are manufactured into a whole and cannot be disassembled. The photosensitive device is arranged below the grating, laid on the lower layer of the micro-fluidic chip, and solidified inside the micro-fluidic chip at the high temperature.

Description

A kind of auto-correlation speed detection device for cell screening
Technical field
The present invention relates to biomedical technology, be specially a kind of auto-correlation speed detection device for cell screening.
Background technology
Cell is the elementary cell of vital movement, is the basis of life science and biomedical research.The root problem of study of disease and cancer is studied cell exactly, because be the generation that the irregular change of various cells causes disease and cancer.But the size of cell is generally at micron order, be difficult to observe and operation, all the time, the screening of cell with separates the research and development of always perplexing cell, and it is just more difficult to detect the speed of cell.Current, measure cell movement speed and mainly contain fluorescence labeling observation and image method.Although these methods can realize observation of cell, measuring speed, also there are a lot of deficiencies in these methods.Fluorescence labeling observation is a kind of the most general method, and the fluorescence that this method adds has produced impact to the living environment of cell, has disturbed the normal life of cell, may cause cell death or the change of cytoactive.Although image method can not affect the activity of cell, image method real-time is not very desirable, can not reflect fast the flow velocity of cell, and this screens at a high speed cell is a very large technical bottleneck.
Summary of the invention
For the deficiencies in the prior art, the technical matters that quasi-solution of the present invention is determined is that a kind of auto-correlation speed detection device for cell screening is provided.This device can be good at measuring the speed of screened cell in micro-fluidic chip passage, has detection speed fast, and intelligent degree is high, separates the feature such as completely.
The technical scheme that the present invention solve the technical problem is, design a kind of auto-correlation speed detection device for cell screening, it is characterized in that this device comprises micro-fluidic chip and the design and installation cylindrical microlens array in micro-fluidic chip, spheroidal microlens array, microchannel, sensor devices, slit, grating, grating pickup groove and laser beam, micro-fluidic chip is the small laboratory of being made up of PDMS, the microchannel that need to be processed with by design in the inside of micro-fluidic chip, below the plane of microchannel, one deck is being inlayed spheroidal microlens array, under spheroidal microlens array, it is grating, in the middle of grating, there are 2 slits of design pitch and width, grating is fixed on micro-fluidic chip the inside with pickup groove, and make one with micro-fluidic chip, non-dismountable, below grating, be sensor devices, sensor devices is laid in the lower floor of micro-fluidic chip, and process hot setting is in micro-fluidic chip the inside.
Compared with prior art, the present invention proposes auto-correlation speed detection device and has well overcome the deficiency of existing sniffer, make auto-correlation speed detection device the flowing velocity of cell can be detected quickly and accurately, to high-flux cell, screening is a good technical support for this, and auto-correlation speed detection device is to be made on micro-fluidic chip, become one with chip, meet miniaturization requirement, be easy to carry and actual use.
Brief description of the drawings
Fig. 1 is the making schematic flow sheet (upward view of bottom of device) of the present invention for a kind of embodiment of auto-correlation speed detection device of cell screening.
Fig. 2 is the grating fixed slot configurations schematic diagram (upward view of bottom of device) of the present invention for a kind of embodiment of auto-correlation speed detection device of cell screening.
Fig. 3 is optical grating construction and the fixed slot configurations schematic diagram (upward view of bottom of device) of the present invention for a kind of embodiment of auto-correlation speed detection device of cell screening.
System Working Principle schematic diagram (front view under device normal operating conditions) when Fig. 4 is the present invention for the laser guide cell of a kind of embodiment of auto-correlation speed detection device of cell screening.
Specific embodiments
Further describe the present invention below in conjunction with embodiment and accompanying drawing thereof.
The auto-correlation speed detection device for cell screening of the present invention's design (is called for short device, referring to Fig. 1-4), it is characterized in that this device comprises micro-fluidic chip 3 and the design and installation cylindrical microlens array 1 in micro-fluidic chip 3, spheroidal microlens array 2, microchannel 4, sensor devices 5, slit 6, grating 7, grating pickup groove 8 and laser beam 9, micro-fluidic chip 3 is the small laboratories of being made up of PDMS, the microchannel 4 that need to be processed with by design in the inside of micro-fluidic chip 3, below 4 planes of microchannel, one deck is being inlayed spheroidal microlens array 2, it under spheroidal microlens array 2, is grating 7, in the middle of grating 7, there are 2 slits 6 of design pitch S and width, grating 7 use pickup grooves 8 are fixed on micro-fluidic chip 3 the insides, and make one with micro-fluidic chip 3, non-dismountable, below grating 7, be sensor devices 5, sensor devices 5 is laid in the lower floor of micro-fluidic chip 3, and process hot setting is in micro-fluidic chip 3 the insides.
Described microchannel 4 is etched in micro-fluidic chip 3 by designing requirement, and the diameter of microchannel 4 is greater than cell dia, inlay column type microlens array 1 in the lower floor of microchannel 4, the diameter of column type microlens array 1 is 50 microns, and oneself height is 35 microns, and the horizontal interval between column type microlens array 1 is 50 microns, described cylindrical microlens array 1 is to be made up of polymethyl methacrylate (PMMA), through 165 DEG C, after the slight baking of 10 minutes, bonding being embedded on micro-fluidic chip 3, the other end becomes spheroidal microlens array 2, its Main Function is regular being irradiated on grating 7 of reflected light 11 producing on cell 10 being irradiated to, slit 6 width on grating 7 are 10 microns, slit 6 is apart from 25 microns of the distances of spheroidal microlens array 2, distance between two slits 6 is S, it below slit 6, is sensor devices 5, sensor devices 5 is to be coated in micro-fluidic chip 3 the insides, coating thickness is 2.5 microns.
The preparation method of apparatus of the present invention embodiment is mainly: in 3 li of processing microchannels 4 of micro-fluidic chip, inlaying diameter at the bottom of microchannel 4 is 50 microns, high 35 microns, level interval is the cylindrical microlens array 1 of 50 microns, through baking in 165 DEG C, 10 minutes, it is 50 microns that the lenticule of these cylindrical microlens arrays 1 forms diameter gradually, the spheroidal microlens array 2 of high 30 microns, spheroidal microlens array 2 is the grating 7 with slit 6 below, distance between two gaps 6 is S, and grating 7 lower floors are the sensor devices 5 that apply.
Apparatus of the present invention, for the auto-correlation speed detection of the microchannel 4 inner cell screenings of micro-fluidic chip 3, have fast, simply, automatically measure the feature of cell speed.
Principle of work and the process of apparatus of the present invention are: cell 10 in the microchannel 4 of micro-fluidic chip 3 because the radiation of laser 9 moves, in moving process, the light being irradiated on cell 10 can light shine on spheroidal microlens array 2 due to reflex, thereby generate scattered beam 11, the movement velocity difference of different cells 10 under laser 9 effects, so the scattered beam 11 generating is also different, thereby it is also different to enter 2 light in slit 6, and then the light that sensor devices 5 is experienced is also different, the light that enters slit 6 converts electric signal to by A/D capture card after sensor devices 5, electric signal is input on outer relay part computer, form 2 similar waveforms, can calculate the time t of light through slit 6 according to waveform, by the distance S of described two slits 6 and the time t of light process slit 6, can calculate the travelling speed of cell again.
The present invention does not address part and is applicable to prior art.

Claims (3)

1. the auto-correlation speed detection device for cell screening, it is characterized in that this device comprises micro-fluidic chip and the design and installation cylindrical microlens array in micro-fluidic chip, spheroidal microlens array, microchannel, sensor devices, slit, grating, grating pickup groove and laser beam, micro-fluidic chip is the small laboratory of being made up of PDMS, the microchannel that need to be processed with by design in the inside of micro-fluidic chip, below the plane of microchannel, one deck is being inlayed spheroidal microlens array, under spheroidal microlens array, it is grating, in the middle of grating, there are 2 slits of design pitch and width, grating is fixed on micro-fluidic chip the inside with pickup groove, and make one with micro-fluidic chip, non-dismountable, below grating, be sensor devices, sensor devices is laid in the lower floor of micro-fluidic chip, and process hot setting is in micro-fluidic chip the inside.
2. the auto-correlation speed detection device for cell screening according to claim 1, it is characterized in that described spheroidal microlens array is to be made up of cylindrical microlens array, two cylindrical microlens array level intervals are 25-65 micron, the height of spheroidal microlens array is 15-45 micron, its level interval is 15 to 65 microns, below spheroidal microlens array, 10-25 micron place is grating, the width of slit is 10 microns, and sensor devices is to apply to be solidificated in micro-fluidic chip the inside.
3. the auto-correlation speed detection device for cell screening according to claim 1, the coating thickness that it is characterized in that described sensor devices is 2.5 microns.
CN201410187981.5A 2014-05-07 2014-05-07 Autocorrelation speed detection device for cell screening Active CN103926421B (en)

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Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN108993620A (en) * 2018-05-31 2018-12-14 京东方科技集团股份有限公司 Micro-fluidic chip and micro-fluidic system
CN113740942A (en) * 2021-08-11 2021-12-03 广州先进技术研究所 Micro-lens array grating and preparation method and application thereof
CN114100708A (en) * 2021-11-15 2022-03-01 矽翔微机电系统(上海)有限公司 Microfluid concentration sensing chip and microfluid characteristic measuring device

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CN1737536A (en) * 2004-08-18 2006-02-22 深圳大学 Five-dimensional fluorescent microscope imaging technique
US20060090573A1 (en) * 2004-11-01 2006-05-04 Foss John F Thermal transient anemometer having sensing cell assembly
WO2010092728A1 (en) * 2009-02-16 2010-08-19 コニカミノルタオプト株式会社 Speed measuring device
CN101903532A (en) * 2008-03-24 2010-12-01 株式会社尼康 Method for analyzing image for cell observation, image processing program, and image processing device
CN101915855A (en) * 2010-07-30 2010-12-15 天津大学 Method and device for detecting flying speeds of particles in cytometer by using secondary light-scattering method

Patent Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN1737536A (en) * 2004-08-18 2006-02-22 深圳大学 Five-dimensional fluorescent microscope imaging technique
US20060090573A1 (en) * 2004-11-01 2006-05-04 Foss John F Thermal transient anemometer having sensing cell assembly
CN101903532A (en) * 2008-03-24 2010-12-01 株式会社尼康 Method for analyzing image for cell observation, image processing program, and image processing device
WO2010092728A1 (en) * 2009-02-16 2010-08-19 コニカミノルタオプト株式会社 Speed measuring device
CN101915855A (en) * 2010-07-30 2010-12-15 天津大学 Method and device for detecting flying speeds of particles in cytometer by using secondary light-scattering method

Cited By (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN108993620A (en) * 2018-05-31 2018-12-14 京东方科技集团股份有限公司 Micro-fluidic chip and micro-fluidic system
CN108993620B (en) * 2018-05-31 2021-01-22 京东方科技集团股份有限公司 Microfluidic chip and microfluidic system
CN113740942A (en) * 2021-08-11 2021-12-03 广州先进技术研究所 Micro-lens array grating and preparation method and application thereof
CN113740942B (en) * 2021-08-11 2022-08-16 广州先进技术研究所 Micro-lens array grating and preparation method and application thereof
CN114100708A (en) * 2021-11-15 2022-03-01 矽翔微机电系统(上海)有限公司 Microfluid concentration sensing chip and microfluid characteristic measuring device

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